A Serological Study Of Some Cauliflower Mosaic Virus Isolates

ABSTRACT

Enzyme-linked immunosorbent assay (ELISA) was used successfully to

detect cauliflower mosaic virus (CaMV) in crude leaf extracts.

Small serological differences between CaMV isolates could be shown

by ELISA and serum cross-absorption. Serological reactivity of

CaMV was found to depend on the proteolytic degradation state of

the virus coat protein so making it impossible to establish defi=

nite serological relationships among the virus isolates tested.

Proteolysis during purification of CaMV could not be entirely

eliminated.

The coat protein of CaMV was shown to be glycosylated by the specific

binding of labelled Concanavalin A. The role of carbohydrate residues

in CaMV serological reactivity was evaluated.