________________________________(Received: 08:03:2017; Accepted: 05:04:2017) _______________________
Abstract
Lipase from Aspergillus flavus was purified in a single step purification using MnFeO4 magnetic nano particles to achieve a 20.53- fold purification with specific activity of 11.29 U/mg and a 59% recovery yield. SDS-PAGE of lipase showed a single pure band with corresponding molecular weight of 35 kDa. The optimal temperature and pH for the enzyme activity were 45°C and 7.0 respectively. Addition of olive oil (1 %w/v) enhanced pH stability of the lipase with 86% residual activity at pH 7.0 after 6 h of incubation while the lipase was thermostable with 79% residual activity after 4 h of incubation. The enzyme activity was enhanced by Ca2+, Mg2+ and Mn2+ while the presence of Cu2+, Co2+ and Zn2+ exhibited inhibitory effect on the enzyme. The study therefore presented lipase from Aspergillus flavus PW2961 with potential in industrial and biotechnological application.
Key words: Lipase, purification, Aspergillus flavus PW2961, magnetic nanoparticles.____
Adebayo, O. (2018). Purification and Characterization of Lipase from Aspergillus flavus PW2961 using Magnetic Nanoparticles. Afribary. Retrieved from https://tracking.afribary.com/works/adebayo-o-s-journal
Adebayo, Oluwatobi "Purification and Characterization of Lipase from Aspergillus flavus PW2961 using Magnetic Nanoparticles" Afribary. Afribary, 03 May. 2018, https://tracking.afribary.com/works/adebayo-o-s-journal. Accessed 22 Nov. 2024.
Adebayo, Oluwatobi . "Purification and Characterization of Lipase from Aspergillus flavus PW2961 using Magnetic Nanoparticles". Afribary, Afribary, 03 May. 2018. Web. 22 Nov. 2024. < https://tracking.afribary.com/works/adebayo-o-s-journal >.
Adebayo, Oluwatobi . "Purification and Characterization of Lipase from Aspergillus flavus PW2961 using Magnetic Nanoparticles" Afribary (2018). Accessed November 22, 2024. https://tracking.afribary.com/works/adebayo-o-s-journal