Ameliorative Potential Of Psidium Guajava Leaf Ethanol Extract On Paraquat Induced Toxicity And Oxidative Stress In Albino Rats

ABSTRACT

The ameliorative potential of Psidium guajava leaf ethanol extract on Paraquat induced toxicity and oxidative stress in Albino Rats were investigated. Thirty (30) healthy male wistar rats were divided into five groups of six animals each. Group 1 served as the normal control (NC) and received normal saline, Group 2 (Intoxicated control) received 3.5mg/kg of paraquat. Group 3 (PG200) received 200mg/kg PG leaf extract + 3.5mg/kg PQ, Group 4 (PG400) received 400mg/kg PG leaf extract + 3.5mg/kg PQ, Group 5 (PG800) received 800mg/kg PG leaf extract + 3.5mg/kg PQ. Extract was administered daily for thirty(30) days using normal saline as vehicle. Animals received 3.5mg/kg PQ intraperitoneally on the 15th day of experiment. Alanine transaminase (ALT), Aspartate transaminase (AST), Total protein, Albumin, and Globulin were estimated to assess hepatoprotection, while Electrolytes, Urea and Creatinine concentrations were estimated to investigate the protective effects of PG on Kidney functions. Malondialdehyde concentration was estimated to assess peroxidation. Glutathione (GSH) concentration was estimated to assess non-enzymatic antioxidant status. Activities of antioxidant enzymes such as superoxide dismutase (SOD) and Catalase (CAT) were further used to assess antioxidant capacity of PG against PQ-induced oxidative damage. Haematological indices were evaluated to assess the effect of PG on haemopoesis in the intoxicated animals. Concentration of the reproductive hormones- Follicle Stimulating hormone(FSH), Leutinizing hormone(LH), Testosterone and prolactin were estimated to determine reproductive health. Results showed that there was a significant increase in ALT & AST activities, Serum Sodium, Urea and Creatinine concentrations, Albumin/Globulin ratio, MDA, WBC-T, and lymphocytes following PQ exposure at (p