Analysis Of Bactericidal And Bacteriostatic Effect Of The Crude Extract Of Scent Leaf (Ocimum Gratissimum) on Escherichia Coli And Staphylococcus Aureus

Overview
Cite Work

ABSTRACT

The antibacterial activity of the extract of Ocimum gratissimum was determined against Escherichia coli and Staphylococcus aureus. The phytochemical analysis of the leaf extract revealed the presence of bioactive bases responsible for antibacterial property, such as Saponin, Alkanoid, Flavonoid, Tanin and Steroid. The methanolic extraction of the active ingredient of the leaves was carried out using the method as described by Fatope, (1993). The minimum inhibitory concentration (MIC) was 12.5mg/ml for E. coli and 25mg/ml for S. aureus and also had minimum bactericidal concentration of 6.25mg/ml for E. coli and 12.5mg/ml for S. aureus. The concentration 1.0mg/disc inhibited the isolates with highest diameter zone of inhibition as 15mm for E. coli and 13mm for S. aureus while the lowest diameter zone of inhibition as 8mm for E. coli and 7mm for S. aureus were recorded at concentration of 0.1mg/disc. Statistical analysis of the extract showed no significant difference (P> 0.05) the test isolates were sensitive to leaf extract of Ocimum gratissimum which implies that the leaf extract of Ocimum gratissimum can serve as a source of therapeutic agent and posses antibacterial properties.

TABLE OF CONTENTS

DECLARATION

CERTIFICATION

ACKNOWLEDMENT

ABSTRACT

TABLE OF CONTENTS

CHAPTER ONE

1.0 INTRODUCTION

1.1 Statement Of The Problem

1.2 Aim And Objectives

CHAPTER TWO

2.0 LITERATURE REVIEW

2.1 Importance of Medicinal Plants

2.2 Medicinal Plants And Antimicrobial Agents

2.3 Other Uses of Medicinal Plants

2.4 An Overview: Ocimum gratissimum:

2.4.1 Scientific classification

2.5 Verification of Ocimum gratissimum

2.6 Uses of Ocimum gratissimum

CHAPTER THREE

3.0 MATERIAL AND METHODS

3.1 Collection Of Plant Materials

3.2 The Test Organisms

3.3 Identification of Bacterial Isolates

3.3.1 Macroscopy

3.3.2 Gram Staining and Microscopy

3.3.2.1 Gram staining Procedures

3.3.3 Biochemical Test

3.3.3.1 Coagulase Test

3.3.3.2 Urease Test

3.3.3.3 Indole Test

3.3.3.4 Citrate Test

3.3.3.5 Catalase Test

3.3.3.6 Motility test

3.3.3.7 Kligler Iron Agar (KIA)

3.4 Extraction procedure

3.5 Preparation of Sensitivity Discs

3.6 Preparation of Sensitivity Discs with Methanolic Extracts Of Ocimum gratissimum

3.7 Phytochemical Screening

3.7.1 Test for saponin

3.7.2 Test for steroids (liberman acid test)

3.7.3 Test for tannins

3.7.4 Test for flavonoids

3.7.5 Test for alkaloids

3.8 Bioassay Procedure

3.9 Determination Of Minimum Inhibitory Concentration (MIC)

3.10 Determination Of Minimum Bactericidal Concentration (MBC)

CHAPTER FOUR

4.0 RESULTS

CHAPTER FIVE

5.0 DISCUSSION

5.1 Conclusion

5.2 Recommendation

REFERENCES

APPENDIX 1

APPENDIX 2

Read Download

Analysis Of Bactericidal And Bacteriostatic Effect Of The Crude Extract Of Scent Leaf (Ocimum Gratissimum) on Escherichia Coli And Staphylococcus Aureus

Related Works