ABSTRACT
The present study examined the total, membrane bound and soluble HLA-G in infected and
uninfected placentas using quantitative real time PCR, ELISA and immunohistochemistry. The
total HLA-G transcripts differed significantly (p = 0.009) between infected and uninfected
primigravidae. The membrane bound HLA-G transcripts were significantly more abundant than
the soluble HLA-G in infected placentas (p = 0.04) in all mothers. At the protein level infected
placentas had higher mean levels of soluble HLA-G than uninfected and naïve sera. Soluble
HLA-G concentration differed significantly between infected and uninfected primigravidae with
a p of 0.001. Immunohistochemistry study demonstrated intense staining of HLA-G in infected
placentas, with localization of membrane bound HLA-G protein in the syncytiotrophoblasts and
near immune cells, when compared to uninfected placentas. Soluble HLA-G localized in the
intervillous spaces and in the lumen of the stroma of forming fetal blood vessels. Soluble HLAG
concentrations in infected primigravidae and secundagravidae were negatively correlated to
birth weight. Conversely, the concentration in infected multigravidae was positively correlated to
birth weight and maternal age. Maternal age and the concentration of soluble HLA-G correlated
negatively in all parities in a physiological pregnancy. However, in infected primigravidae and
multigravidae the maternal age and soluble HLA-G correlated positively. The findings of this
study indicate that there are high levels of HLA-G in infected placentas from all parities
compared to the uninfected placentas. However, the levels of HLA-G in infected primigravidae
are significantly higher than in uninfected with p value of 0.001. These results shows that HLAG
is involved in mediating anti-inflammatory response in P. falciparum infected placentas. The
study findings suggest that interventions during pregnancy malaria should promote expression of
HLA-G in the placenta to check down the effects of the inflammatory infiltrates in the P. falciparum infected placenta.
, M & KARAU, G (2021). Analysis Of Total Hla-G Levels And Its Isoforms In Placental Malaria. Afribary. Retrieved from https://tracking.afribary.com/works/analysis-of-total-hla-g-levels-and-its-isoforms-in-placental-malaria
, MURIIRA and GEOFFREY KARAU "Analysis Of Total Hla-G Levels And Its Isoforms In Placental Malaria" Afribary. Afribary, 14 May. 2021, https://tracking.afribary.com/works/analysis-of-total-hla-g-levels-and-its-isoforms-in-placental-malaria. Accessed 09 Nov. 2024.
, MURIIRA, GEOFFREY KARAU . "Analysis Of Total Hla-G Levels And Its Isoforms In Placental Malaria". Afribary, Afribary, 14 May. 2021. Web. 09 Nov. 2024. < https://tracking.afribary.com/works/analysis-of-total-hla-g-levels-and-its-isoforms-in-placental-malaria >.
, MURIIRA and KARAU, GEOFFREY . "Analysis Of Total Hla-G Levels And Its Isoforms In Placental Malaria" Afribary (2021). Accessed November 09, 2024. https://tracking.afribary.com/works/analysis-of-total-hla-g-levels-and-its-isoforms-in-placental-malaria