Abstract The role of enzyme engineering in biotechnol-
ogy, biological and pharmaceutical process cannot be over
emphasized. This study compared the adsorption of
digestives enzymes; amylase, protease and lipase on to Zn-
ferrite (ZnFe2O4). The metal ferrite was synthesized via a
sol–gel technique and characterized with scanning electron
microscopy (SEM), X-ray diffraction (XRD), Electron
paramagnetic resonance (EPR) and Fourier transform
infrared spectroscopy (FTIR). The adsorption was studied
in a batch process and the data were subjected to kinetics
and isotherm models. Characterization shows that the
particle has a nanoporous structure, with pore sizes of
about 5.4 nm and good magnetic properties. The FTIR data
showed the presence of M–O bond, which is a character-
istic of metal ferrites. The adsorption of the amylase, lipase
and protease on ZnFe2O4 follow first-order kinetic model
with rate constants increasing with concentration. The
maximum adsorption capacities as revealed by the gener-
alized adsorption isotherms are 7.20, 42.90 and
22.24 mg g-1 for amylase, lipase and protease, respec-
tively, with cooperative binding. The Dubinin–Radushke-
vich model gave the maximum adsorption energies, E of
3.74 kJ mol-1 for amylase, 2.01 kJ mol-1 for lipase and
1.51 kJ mol-1 for the protease adsorption, showing that the
process is physisorption dominated. The isotherms fit the
adsorption data in the order of Freundlinch[General-
ized [Guggenheim–Anderson–de Boer[Tempkin
isotherm[Dubinin–Radushkevich. Thermodynamic study
revealed a spontaneous adsorption process with increased
entropy. ZnFe2O4, therefore, is a very good adsorbent for
the purification of enzymes and can be used as a supporter
for enzymatic process that required immobilization of the
enzymes.
Keywords Adsorption -
Enzymes -
Zn-ferrite -
Kinetic and
isotherm
Introduction
Adsorption of proteins on solid surfaces
Adebayo, T. & Adebayo, O. (2019). Comparative adsorption of amylase, protease and lipase on ZnFe2O4: kinetics, isothermal and thermodynamics studies. Afribary. Retrieved from https://tracking.afribary.com/works/comparative-adsorption-of-amylase-protease-and-lipase-on-znfe2o4-kinetics-isothermal-and-thermodynamics-studies-abideen-idowu-adeogun1-sarafadeen-olateju-kareeem2
Adebayo, Tobi, and Oluwatobi Adebayo "Comparative adsorption of amylase, protease and lipase on ZnFe2O4: kinetics, isothermal and thermodynamics studies" Afribary. Afribary, 30 Sep. 2019, https://tracking.afribary.com/works/comparative-adsorption-of-amylase-protease-and-lipase-on-znfe2o4-kinetics-isothermal-and-thermodynamics-studies-abideen-idowu-adeogun1-sarafadeen-olateju-kareeem2. Accessed 21 Nov. 2024.
Adebayo, Tobi, and Oluwatobi Adebayo . "Comparative adsorption of amylase, protease and lipase on ZnFe2O4: kinetics, isothermal and thermodynamics studies". Afribary, Afribary, 30 Sep. 2019. Web. 21 Nov. 2024. < https://tracking.afribary.com/works/comparative-adsorption-of-amylase-protease-and-lipase-on-znfe2o4-kinetics-isothermal-and-thermodynamics-studies-abideen-idowu-adeogun1-sarafadeen-olateju-kareeem2 >.
Adebayo, Tobi and Oluwatobi Adebayo . "Comparative adsorption of amylase, protease and lipase on ZnFe2O4: kinetics, isothermal and thermodynamics studies" Afribary (2019). Accessed November 21, 2024. https://tracking.afribary.com/works/comparative-adsorption-of-amylase-protease-and-lipase-on-znfe2o4-kinetics-isothermal-and-thermodynamics-studies-abideen-idowu-adeogun1-sarafadeen-olateju-kareeem2