Abstract/Overview
The contribution of appropriate inoculum strains to more efficient nitrogen fixation by legumes has been difficult to assess due to the laborious nature of the assays involved in assessing establishment of inoculum strains in the field. The use of marker genes, in particular the GUS system, changes this, making it possible to assess occupancy by the inoculum strain in large numbers of nodules on whole root systems. Here we used the GUS system to evaluate the competitive ability of two rhizobial strains, Rhizobium leguminosarum bv. phaseoli strain Kim5 and R. tropici strain CIAT899 in two soil types from Kenya. The results confirm that Kim5 is a highly competitive strain, forming 86% of the nodules in a near-neutral pH soil. Although the competitiveness of CIAT899 is enhanced in an acid (pH 4.5) soil it still only formed 35% of the nodules. There were no differences between inoculum strains in their efficiency of nitrogen fixation in either soil type, and virtually no N2-fixation occurred in the acid soil due to the lack of tolerance of the Phaseolus genotype to soil acidity.
Wilson, A (2024). Competition in Kenyan soils between Rhizobium leguminosarum biovar phaseoli strain Kim5 and R. tropici strain CIAT899 using the gusA marker gene. Afribary. Retrieved from https://tracking.afribary.com/works/competition-in-kenyan-soils-between-rhizobium-leguminosarum-biovar-phaseoli-strain-kim5-and-r-tropici-strain-ciat899-using-the-gusa-marker-gene
Wilson, Anyango "Competition in Kenyan soils between Rhizobium leguminosarum biovar phaseoli strain Kim5 and R. tropici strain CIAT899 using the gusA marker gene" Afribary. Afribary, 04 Jun. 2024, https://tracking.afribary.com/works/competition-in-kenyan-soils-between-rhizobium-leguminosarum-biovar-phaseoli-strain-kim5-and-r-tropici-strain-ciat899-using-the-gusa-marker-gene. Accessed 22 Dec. 2024.
Wilson, Anyango . "Competition in Kenyan soils between Rhizobium leguminosarum biovar phaseoli strain Kim5 and R. tropici strain CIAT899 using the gusA marker gene". Afribary, Afribary, 04 Jun. 2024. Web. 22 Dec. 2024. < https://tracking.afribary.com/works/competition-in-kenyan-soils-between-rhizobium-leguminosarum-biovar-phaseoli-strain-kim5-and-r-tropici-strain-ciat899-using-the-gusa-marker-gene >.
Wilson, Anyango . "Competition in Kenyan soils between Rhizobium leguminosarum biovar phaseoli strain Kim5 and R. tropici strain CIAT899 using the gusA marker gene" Afribary (2024). Accessed December 22, 2024. https://tracking.afribary.com/works/competition-in-kenyan-soils-between-rhizobium-leguminosarum-biovar-phaseoli-strain-kim5-and-r-tropici-strain-ciat899-using-the-gusa-marker-gene