Abstract
Background: Cytokines play an important role in signaling the immune system to build an adequate immune response against HIV. HIV distorts the balance between pro and anti-inflammatory cytokines causing viral replication. Highly active antiretroviral treatment (HAART) acts by trying to restore pro and anti-inflammatory cytokine balance. It is not clear how HAART non-adherence influences circulating cytokine levels. This study therefore determined cytokine levels in HAART non-adherent individuals.
Methods: This cross-sectional study recruited 163 participants (51 controls, 23 HIV-1+ HAART naive, 28 HAART-adherent 6 months, 19 HAART-adherent 12 months and 42 HAART non-adherent). Cytokines were analyzed by ELISA while CD4 T cells determined in 3.0 µl of whole blood using BD FACSCaliburTM and viral load in 0.2ml plasma sample using Abbott Molecular m2000sp sample preparation and m2000rt real-time amplification and detection systems (Abbott Molecular Inc., Illinois, USA) according to the manufacturer's methods.
Results: IL-4, IL-6, IL-10, TNF-α and TGF-β were significantly elevated in HIV-1 HAART non-adherent compared with HIV-1 HAART adherent and healthy controls P
<div>Shaviya, < (2024). Cytokine profiles in highly active antiretroviral treatment non-adherent, adherent and naive HIV-1 infected patients in Western Kenya. Afribary. Retrieved from https://tracking.afribary.com/works/cytokine-profiles-in-highly-active-antiretroviral-treatment-non-adherent-adherent-and-naive-hiv-1-infected-patients-in-western-kenya
<div>Shaviya, <div>Musa "Cytokine profiles in highly active antiretroviral treatment non-adherent, adherent and naive HIV-1 infected patients in Western Kenya" Afribary. Afribary, 04 Jun. 2024, https://tracking.afribary.com/works/cytokine-profiles-in-highly-active-antiretroviral-treatment-non-adherent-adherent-and-naive-hiv-1-infected-patients-in-western-kenya. Accessed 14 Sep. 2024.
<div>Shaviya, <div>Musa . "Cytokine profiles in highly active antiretroviral treatment non-adherent, adherent and naive HIV-1 infected patients in Western Kenya". Afribary, Afribary, 04 Jun. 2024. Web. 14 Sep. 2024. < https://tracking.afribary.com/works/cytokine-profiles-in-highly-active-antiretroviral-treatment-non-adherent-adherent-and-naive-hiv-1-infected-patients-in-western-kenya >.
<div>Shaviya, <div>Musa . "Cytokine profiles in highly active antiretroviral treatment non-adherent, adherent and naive HIV-1 infected patients in Western Kenya" Afribary (2024). Accessed September 14, 2024. https://tracking.afribary.com/works/cytokine-profiles-in-highly-active-antiretroviral-treatment-non-adherent-adherent-and-naive-hiv-1-infected-patients-in-western-kenya