ABSTRACT
Banana is an important crop in East Africa. In the tropics, banana and plantains are a major
source of carbohydrate for about 400 million people of whom 200 million are from East
Africa. In Kenya, the banana serves both as a subsistence crop as well as a cash crop. The
production of banana in Kenya is however constrained by viral diseases especially the
Banana Streak Disease (BSD) which is caused by the Banana Streak Virus (BSV). Breeding
between A and B-genomes containing banana have been significantly constrained by BSV
due to activation of the integrated viral sequences in the genome of the interspecific hybrids.
The episomal DNA viral particles are thought to be transmitted by several mealy-bug species
but there are no reports on the transmission of the BSV by other viral insect vectors. This
study aimed at elucidating the mode of transmission of BSV by mealy-bug species in Kenya
and identifying any other putative vectors of BSV in samples collected from infected banana
plantations. The mealy-bugs were reared on pumpkin fruits in black cages and were used in
the virus transmission cycle experiments. The instars of the Paracoccus burnerae (mealybug)
species were fed on BSV infected plants to acquire the virus and on the healthy plantlets
for inoculation. The effect of the acquisition period on the transmission of the BSV, the latent
period and the retention period of the BSV by the mealy-bug species were determined using
Rolling Circle Amplification (RCA). In addition, leafhoppers and banana aphids collected
from the infected banana plantations were assayed for the presence of BSV using RCA. RCA
products were assayed by electrophoretic analysis. Principal characteristics of the modes of
virus transmission by insects were used to conclude the mode of transmission of the BSV by
mealy-bugs. Results from this study revealed that the P. burnerae is able to acquire the virus
and transmit it after a minimum of six hours of acquisition access time. The virus had no
latent period in the vector though it could be retained in the vector for four days after
acquisition feeding, characteristics of semi-persistent mode of transmission. In addition, the
results revealed that Banana aphids and leafhoppers are not potential vectors of BSV.
Viruliferous mealy-bugs should be reduced by destroying BSV infected materials. Greater
understanding of the transmission of BSV will contributed immensely to the development of control strategies for banana streak disease
MWANGANGI, M (2021). Development Of A Novel Dna Isolation Protocol And Determination Of The Transmission Mode Of Banana Streak Virus By Putative Vectors In Kisii. Afribary. Retrieved from https://tracking.afribary.com/works/development-of-a-novel-dna-isolation-protocol-and-determination-of-the-transmission-mode-of-banana-streak-virus-by-putative-vectors-in-kisii
MWANGANGI, MUTURI "Development Of A Novel Dna Isolation Protocol And Determination Of The Transmission Mode Of Banana Streak Virus By Putative Vectors In Kisii" Afribary. Afribary, 13 May. 2021, https://tracking.afribary.com/works/development-of-a-novel-dna-isolation-protocol-and-determination-of-the-transmission-mode-of-banana-streak-virus-by-putative-vectors-in-kisii. Accessed 24 Nov. 2024.
MWANGANGI, MUTURI . "Development Of A Novel Dna Isolation Protocol And Determination Of The Transmission Mode Of Banana Streak Virus By Putative Vectors In Kisii". Afribary, Afribary, 13 May. 2021. Web. 24 Nov. 2024. < https://tracking.afribary.com/works/development-of-a-novel-dna-isolation-protocol-and-determination-of-the-transmission-mode-of-banana-streak-virus-by-putative-vectors-in-kisii >.
MWANGANGI, MUTURI . "Development Of A Novel Dna Isolation Protocol And Determination Of The Transmission Mode Of Banana Streak Virus By Putative Vectors In Kisii" Afribary (2021). Accessed November 24, 2024. https://tracking.afribary.com/works/development-of-a-novel-dna-isolation-protocol-and-determination-of-the-transmission-mode-of-banana-streak-virus-by-putative-vectors-in-kisii