Effects of Oral Contraceptive Pills Intake on Factor V Leiden Gene Mutation among Sudanese Women

Abstract

This case control study was conducted in Khartoum state – Sudan during the period from April

to November 2018. The study aimed to study effect of oral contraceptive pill on factor V leiden.

The Study included 50 women who use oral contraceptive as cases and 50 women don’t use oral

contraceptive pill as control, all subject were informed verbally about the study and approved for

participation. From each participant 3 ml of venous blood collected in EDTA container then

sample prepared for PCR (Eppendorf, master cycler) to analyze factor V Leiden gene mutation

in exon 10 by using specific primer, the wild-type primer is 5, GGACAAAATACCT

GTATTCCTC,3 the mutant primer is 5, GGACAAAATAC CTGTATTCCTT,3 and the common

primer is 5,CTTTCA GGCAGGAACAACACC,3.

Result analyzed by SPSS (version 16) computerized program.

The result obtained from cases show that mean of age is30±5.5and it divided into three group

less than 20 with least frequency 4%(2/50), (20-35) was most frequancy80%(40/50) and more

than 35 years has frequancy16%(8/50).

Most case use oral contraceptive pill for more than 1years with frequency 60%(30/50) with mean

2±0.8.

The most frequent oral contraceptive pill use was levonorgestrel 88%(44/50) followed by

desogestrel12%(6/50).

The result show no gene mutation with no significant different between user and non-user oral

contraceptive pill.

Also there no significant association was found between mutation and demographic data as well

as mean of age between user and non-user oral contraceptive pill P.value (P=0.52).

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