ABSTRACT
Microbes capable of degrading atrazine were isolated by traditional enrichment technique from composite soil samples collected from four agricultural farmlands with long history of atrazine use in Nigeria. The estimated atrazine degrading populations from Obasanjo, Ladipo Daniel, Agbowa - Ikosi and Itokin farms were 1.6 x 10* cfu/mg, 8.0 x 10^cfu/mg, 5.0 x 10* cfw/mg and 1.2 x 10 cfu/mg of the soil samples respectively. The soil sample from Obasanjo farm was selected for further studies. Isolation of atrazine-degraders was carried out using a novel in situ enrichment approach with highly porous atrazine- impregnated BioSep beads in both glucose and non-glucose mineral salts media. The beads were impregnated with atrazine at concentrations of 20 mg/l and 200 mg/l plus a water equilibrated control. Degradation of atrazine was detected using high performance liquid chromatography (HPLC). Both bead and soil enriched cultures resulted in complete disappearance of atrazine. Mixed consortia and pure cultures of bacteria were obtained from the two enrichment techniques. It was however, easier and faster to obtain pure cultures with BioSep beads. Community structure of the enrichment cultures were analyzed by Denaturing Gradient Gel Electrophoresis (DGGE). Identification of bands and isolates were carried out by 16 TRNA sequencing. The DGGE analysis of the unenriched control and atrazine enriched beads and soil cultures in both glucose and non-glucose supplements revealed the presence of many bands corresponding to different bacteria.
ELIZABETH, O (2021). Enrichment And Molecular Characteristization Of Atrazine Degrading Actinomycetes Isolated From Polluted Nigerian Commercial Farmlands. Afribary. Retrieved from https://tracking.afribary.com/works/enrichment-and-molecular-characteristization-of-atrazine-degrading-actinomycetes-isolated-from-polluted-nigerian-commercial-farmlands
ELIZABETH, OMOTAYO "Enrichment And Molecular Characteristization Of Atrazine Degrading Actinomycetes Isolated From Polluted Nigerian Commercial Farmlands" Afribary. Afribary, 08 May. 2021, https://tracking.afribary.com/works/enrichment-and-molecular-characteristization-of-atrazine-degrading-actinomycetes-isolated-from-polluted-nigerian-commercial-farmlands. Accessed 22 Nov. 2024.
ELIZABETH, OMOTAYO . "Enrichment And Molecular Characteristization Of Atrazine Degrading Actinomycetes Isolated From Polluted Nigerian Commercial Farmlands". Afribary, Afribary, 08 May. 2021. Web. 22 Nov. 2024. < https://tracking.afribary.com/works/enrichment-and-molecular-characteristization-of-atrazine-degrading-actinomycetes-isolated-from-polluted-nigerian-commercial-farmlands >.
ELIZABETH, OMOTAYO . "Enrichment And Molecular Characteristization Of Atrazine Degrading Actinomycetes Isolated From Polluted Nigerian Commercial Farmlands" Afribary (2021). Accessed November 22, 2024. https://tracking.afribary.com/works/enrichment-and-molecular-characteristization-of-atrazine-degrading-actinomycetes-isolated-from-polluted-nigerian-commercial-farmlands