ABSTRACT
Piper guineense (Schumach) has great potentials for economic exploration because of the
proven use of its medicinal content in human health. The plant material is usually obtained from
thick forests by women who have to walk long distances in the bush to reach it. A technique that
improves its propagation and domestication, such as tissue culture, becomes necessary. This
study was therefore initiated to develop a method for the mass propagation of Piper guineense
seedlings using in vitro regeneration. Plants react differently to media concentrations and
constituents for their in vitro regeneration. Comparative growth of Piper guneensee inoculated
on Murashige and Skoog(MS) medium supplemented with some growth regulators were
investigated. Mature nodes were collected from the medicinal garden of National Centre for
Genetic Resources and Biotechnology (NACGRAB) Ibadan. Nodes of the species were
inoculated onto MS media. The MS media was supplemented with the following concentrations
of growth regulators 0.25, 0.50, 0.75, 1.0mg/1. BAP, the same concentration for KIN and
0.05mg/l of NAA for all replicate. After two weeks of inoculation it was observed that, the
plantlet with BAP 0.50mg/l +NAA 0.0Smg/1 were found contamination-free and sprouted, while
other treatments showed contamination including control. Furthermore, after 7 days the sprouted,
plantlets with treatment of BAP 0.50mg/l +NAA 0.05mg/I were also contaminated, thereby
yielding no positive result. Many repeats of the experiment also yielded contaminated products.
It is therefore recommended that antibiotics should play an important role in the culture medium for P. guineense in order to eliminate deep tissue contaminants.
Tope, O (2021). Establishing A Method For The Mass Propagation Of Piper Guineense (Schumach) Via Tissue Culture.. Afribary. Retrieved from https://tracking.afribary.com/works/establishing-a-method-for-the-mass-propagation-of-piper-guineense-schumach-via-tissue-culture-1
Tope, OKAFOR "Establishing A Method For The Mass Propagation Of Piper Guineense (Schumach) Via Tissue Culture." Afribary. Afribary, 21 May. 2021, https://tracking.afribary.com/works/establishing-a-method-for-the-mass-propagation-of-piper-guineense-schumach-via-tissue-culture-1. Accessed 18 Dec. 2024.
Tope, OKAFOR . "Establishing A Method For The Mass Propagation Of Piper Guineense (Schumach) Via Tissue Culture.". Afribary, Afribary, 21 May. 2021. Web. 18 Dec. 2024. < https://tracking.afribary.com/works/establishing-a-method-for-the-mass-propagation-of-piper-guineense-schumach-via-tissue-culture-1 >.
Tope, OKAFOR . "Establishing A Method For The Mass Propagation Of Piper Guineense (Schumach) Via Tissue Culture." Afribary (2021). Accessed December 18, 2024. https://tracking.afribary.com/works/establishing-a-method-for-the-mass-propagation-of-piper-guineense-schumach-via-tissue-culture-1