EVALUATION OF MICROBIOLOGICAL QUALITY OF TANK WATER AT THE INSTITUTE OF MANAGEMENT AND TECHNOLOGY {IMT} CAMPUS III ENUGU

ABSTRACT
The evaluation of microbiological quality of tank water at the Institute of Management and Technology campus III Enugu was carried out by using ten samples of water obtained from ten different tank waters. For viable plate count for the presence of pathogenic organisms and organisms indicating organic matter pollution, plating technique of pour plates were used, also presence of coliform groups and Escherichia  coli were examined using the Presumptive, Confirmatory and Completed  coliform test techniques. The media used include Nutrient agar, Brilliant green bile agar, Eosin Methylene Blue agar (EMB), MacConkey broth and Brilliant green bile broth. The total viable plate count gave 31 X 10-3 cfu/ml for pathogenic organisms and 21 X 10-3 cfu/ml for organisms indicating organic matter pollution, whereas the presumptive test showed the presence of coliform groups in seven (7) samples of water out of ten. The confirmatory test showed the presence of colform in five (5) samples of water whereas the presence of Escherichia coli was shown in three (3) samples. The completed test result revealed a highest coliform count of 2 cfu in one sample of water while 1cfu was

obtained in three (3) water samples for Escherichia coli. The result indicated the presence of coliform and Escherichia coli but from the

deduction, the microbial load was minimal in all the tests. Therefore, the tank water at campus III of IMT is not fit for drinking though it

can be used for other domestic purposes.


TABLE OF CONTENTS
Title page                                    i
Certification                                    ii
Dedication                                    iii
Acknowledgement                                    iv
Table of contents                                v
List of Tables                                    viii
Abstract                                    ix

CHAPTER ONE
    INTRODUCTION
    Background of Study                            1
    Statement of  the Problem                         2
    Objective of the Study                            3
    Hypothesis                                3
    Significance of the study                        3
    Limitations of the study                        4

CHAPTER TWO
    LITERATURE REVIEW                            5
    Sources of Tank Water                                   6
    Drinking Water                                8
    Drinking Water Standard                                8
    Tank water Contamination                        10
    Tank Water purification/Treatment                        12
    Water pollution Indicator                        15

CHAPTER THREE
    MATERIALS AND METHODS       
    Materials (see appendix)                        18
    Methods                                    18
    3.2.1     Sterilization of Materials                    18
      Collections of Water Samples                18
      Preparation of Culture Media                19
     Dilution of Water Samples                    19
    Examination of Water Samples                   
 Total Viable Plate Counts                    20   
 Bacteriological Examination                21



CHAPTER FOUR
    RESULT                                        25
    Total Viable Plate Counts                        25
    Coliform Test                               
 Presumptive  Test                        26
 Confirmatory Test                        27
 Completed Test                            29
CHAPTER FIVE
DISCUSSION, CONCLUSION AND RECOMMENDATION
     Discussion                                    30
     Conclusion                                    33
     Recommendation                            34

REFERENCES                                        35
APPENDIX                                            37




LIST OF TABLES
Table 1:
Total Viable Plates Count for pathogenic organisms and organisms for organic matter pollution present in the ten samples of water     25

Table 2: Presumptive test for the number of tubes positive for coliform organisms                                            26

Table 3: Confirmatory test for coliform organisms and Escherichia   coli present in the water samples and their most probable number (mpn).     27
Table 4: Completed test for coliform organisms and Escherichia coli.    29