Evaluation Of Plasma Interleukin-10 Levels Among Chronic Hepatitis B Patients Attending Total Lab Care Laboratories In Khartoum State.

ABSTRACT

One of the most important factors playing role in chronic hepatitis B pathogenesis is cytokine release and one of the cytokines with antiinflammatory characteristic is interleukin-10 (IL-10). The aim of the present study was to estimate IL-10 levels and to evaluate the utility of using it as biomarker for monitoring the progression and treatment of chronic hepatitis B infection. Sixty patients with chronic hepatitis B disease confirmed by persistence expression of HBsAg for more than 6 months and 30 healthy controls were included in the study during the period from January to May 2017. Serum IL-10 level was investigated by Enzyme Linked Immunosorbent assay (ELISA) technique and HBV viral load was investigated by Real Time PCR. In the control group, thirty healthy individuals with age group (23-58 years) similar to the patient population were included. Controls and patients groups were compared and data were statistically analyzed. Interleukin-10 levels of the patients was as the following from total of 60 patients 37 who represents (61.6%) of the total patients were in the levels of 1-10 ng/ml, 7 (11.7%) were in the levels of 11-20 ng/ml, 6 (10%) were in the levels of 21-40 ng/ml and 10 (16.7 %) were in the levels of more than 40 ng/ml. Interleukin-10 levels of the controls was as the following from total of 30 controls 26 who represents (86.6%) of the total controls were in the levels of 1-10 ng/ml, 3 (10%) were in the levels of 11-20 ng/ml, 1 (3.4%) were in the levels of 21-40 ng/ml and zero of the controls were in the levels of more than 40 ng/ml. According to HBV viral load the 60 patients had been divided into two groups, the first consists of 41 (68%) patients with HBV DNA level of 6‒2000 IU/mL, the second consists of 19 (32%) patients with HBV DNA level of > 2000 IU/mL. Interleukin-10 levels of the first group was compared with the control group and the levels in chronic hepatitis B group were statistically significantly higher (P=0.01).