Evaluation Of The Performance Of Bacillus Cereus And Bacillus Subtilis As Test Organisms For Assay Of Tetracyclines And Beta-lactams In Chicken Meat

Subscribe to access this work and thousands more

The uncontrolled and unrestricted use of antimicrobials may lead to the accumulation of undesirable drug residues in the treated animals and their products. To avoid adverse effects from antibiotic residue consumption such as drug resistance, regulations such as maximum residue limits (MRLs), acceptable daily intakes (ADIs) for humans and withholding times for pharmacologically active substances have been set. However, in the Kenyan poultry industry there lacks affordable, easy to perform antibiotic residues screening methods. The aim of this study was to evaluate the performance of Bacillus cereus and Bacillus subtilis as test organisms for assay of tetracyclines and beta-lactam antibiotics in chicken meat. Microbiological detection was achieved by agar well diffusion using 24 combinations of the two test bacteria, three medium pH, two poultry organs and two antibiotics. The test bacteria grew optimally at a pH range of 6-7.3. Inhibition zones increased significantly (p

Subscribe to access this work and thousands more