Evaluation Of The Safety And Immunogenicity Of Rift Valley Fever Mp-12 And Armp-12∆Nsm21/384 Vaccine Candidates In Goats (Capra Hircus), Sheep (Ovis Aries) And Calves (Bos Indicus) From Tanza

ABSTRACT

Rift Valley fever virus (RVFV) is an arbovirus that causes Rift Valley fever (RVF), a disease that causes morbidity and mortality in livestock and humans. Among the measures considered, vaccines are the most effective control strategy against this RVF disease. While we have available vaccines, effective vaccines and better routes of vaccination are needed to prevent RVF among livestock and humans. Therefore, the aim of this study was to evaluate the safety and immunogenicity of a live attenuated RVFV MP-12 and a derivative recombinant RVFV arMP-12∆NSm21/384 vaccine using the intramuscular (IM) route of vaccination in Tanzanian calves, and goats. Also, a proof of concept study was conducted to evaluate the safety and immunogenicity of the RVFV arMP-12∆NSm21/384 vaccine using the intranasal (IN) route of vaccination in the same species, as well as sheep. Overall, a total of 61 animals (goats, sheep and calves) aged 6 to 9 months old were used in this study. Twenty five animals, including 5 goats and 3 calves were vaccinated IM with a dose of 1×105 plaque forming units (PFU)/ml of RVF MP-12 and 8 goats and 5 calves were vaccinated with the RVF arMP-12∆NSm21/384 vaccine, and 2 goats and 2 calves received a placebo to serve as controls. Afterward, rectal temperatures were recorded on day 1, 2, 3, 4, 5, 7 then weekly throughout the study. Blood samples were collected on day 14 before vaccination and on the days 0, 3, 5, 7, 14, 21, 28, 35, 70, 84 and 87 post vaccinations (PV). At day 87 PV, all IM vaccinated animals were revaccinated via the IM route with 1×104 PFU/ml of RVF MP-12 vaccine and blood samples were again collected on days 94, 101 and 108 PV. As a proof of concept study, 7 sheep, 10 goats and 10 calves were vaccinated intra-nasally (IN) with 50 µl of 1×105 PFU of arMP-12∆NSm21/384 vaccine and 2 sheep were vaccinated with 100 ul of 1x 105 PFU of the arMP12∆NSm21/384 vaccine, and 7 animals (2 goats, 3 sheep and 2 calves) received a placebo to serve as controls. Rectal temperatures were recorded and blood samples were collected iii 14 days before and on day, 0 immediately before vaccination and on days 3, 5, 7, 14, 21, 28 and 35 PV. Samples collected in both studies on the day -14 before vaccination, day 0 immediately before vaccination, 3 and 5 were tested for viremia by virus isolation attempted in Vero E6 cells and samples collected on days 7, 14, 21, 28, 35, 70, 84, 87, 94, 101 and 108 PV were tested for RVFV neutralizing antibody by the plaque reduction neutralization test (PRNT). None of the animals had detectable viremia and clinical manifestations throughout the study. All IM vaccinated animals and 70% of each species in the IN vaccinated animals had the first detectable antibody on either day 5 or 7 PV, respectively and antibody titers ranged from 1: 10 to 1:40. Afterwards, antibody titers increased and ranged from 1:10 to 1:640 for the IN and 1:40 to 1:640 for IM vaccinated animals. The antibody response was lower for the IN vaccinated animals, but goats that were vaccinated both by the IM and IN routes responded better than other species while calves had the lowest antibody titers. Therefore, these findings demonstrated that the IN route of vaccination is promising for use in place of the IM route to avoid the use of needles that can cause needle stick injuries and the IN route may prove to be a safer and more efficient route of vaccination especially in mass vaccination campaigns. However, based on the preliminary results of this study, the volume of the vaccine dose for IN vaccination may need to be increased from 50 µl to 100 ul per animal to improve the immunogenicity of the vaccine.