ABSTRACT
Pearl millet is a staple food and fodder crop of marginal areas of sub-Saharan Africa, South Asia and the Americas. Although pearl millet is stable food for 70 % of Namibian population, its productivity is very low (low yield, susceptible to diseases/insect and prolonged reproductive cycle) and has a limited genetic diversity. For the purpose of widening gene diversity, developing high yielding and drought resistant pearl millet, the Kangara and Okashana 2 were mutated using Colbalt-60 gamma irradiation source with various dose levels. However, all sixth generation of Okashana 2 putative mutant derivatives used in this study were all produced using 350 Gray while for Kangara mutants were produced using 400 Gray. A total of 32 genotypes including two parental lines were collected from the three agroecological zones (Research Stations). In addition to two parental lines, stratified sampling technique was used to randomly select further 12 samples from 30 putative mutants for Okashana 2 and Kangara that offered higher average grain yields. Such technique used to subsample was applied in order to select putative mutants that shown phenotypically high yielding trait. This study aimed to examine the genetic diversity existing in the Namibian pearl millet putative mutant lines using 19 simple sequence repeats and 23 biochemical markers. Genetic characterization was studied using conventional PCR and separated using gel electrophoresis while biochemical parameters used dumas method (crude protein), fiber extractor (crude fibre, ADF and NDF), fat solvent extractor (fat), ICP spectrophotometer (minerals) and UV/VIS spectrometer (phytate).
SHINDUME, F (2021). Genetic And Biochemical Characterization Of Pearl Millet Mutant Lines Using Simple Sequence Repeat Markers In Namibia. Afribary. Retrieved from https://tracking.afribary.com/works/genetic-and-biochemical-characterization-of-pearl-millet-mutant-lines-using-simple-sequence-repeat-markers-in-namibia
SHINDUME, FILEMON "Genetic And Biochemical Characterization Of Pearl Millet Mutant Lines Using Simple Sequence Repeat Markers In Namibia" Afribary. Afribary, 19 Apr. 2021, https://tracking.afribary.com/works/genetic-and-biochemical-characterization-of-pearl-millet-mutant-lines-using-simple-sequence-repeat-markers-in-namibia. Accessed 22 Nov. 2024.
SHINDUME, FILEMON . "Genetic And Biochemical Characterization Of Pearl Millet Mutant Lines Using Simple Sequence Repeat Markers In Namibia". Afribary, Afribary, 19 Apr. 2021. Web. 22 Nov. 2024. < https://tracking.afribary.com/works/genetic-and-biochemical-characterization-of-pearl-millet-mutant-lines-using-simple-sequence-repeat-markers-in-namibia >.
SHINDUME, FILEMON . "Genetic And Biochemical Characterization Of Pearl Millet Mutant Lines Using Simple Sequence Repeat Markers In Namibia" Afribary (2021). Accessed November 22, 2024. https://tracking.afribary.com/works/genetic-and-biochemical-characterization-of-pearl-millet-mutant-lines-using-simple-sequence-repeat-markers-in-namibia