Physiological And Biological Responses Of Sorghum Bicolor [L.] Moench. To Drought Stress Condition.

ABSTRACT

This study was conducted to ascertain drought tolerance in sorghum genotypes. Seedlings of locally cultivated sorghum were screened for drought tolerance by assessing percentage relative

water content (R WC) after progressive water deficit. Plant biomass, root/shoot ratio, tolerance index, relative water content (RWC) were measured during harvest. Malondialdehyde (MDA),

glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), chlorophyll and praline content were quantified. The four genotypes (DTSYN 11, EC - 3161, RJ - 2020 and BPCH - 6) recorded

high R WC after subsequent evaluation for their physiological response to severe ( 10 days) water stress treatment. All four genotypes maintained R WC above 80% during severe stress treatment. MDA for EC - 3161 was 5% higher and BPCH - 6 were recorded within 5% less in comparison

to their well - watered controls. while GSH, SOD, CAT had a significant difference in RJ - 2020, BPCH - 6 and DTSYN 11. Significant higher chlorophyll content was recorded for both RJ -2020

and EC - 3161 during severe stress. The proline content significantly increased by 14 - fold and 16 - fold in RJ - 2020 and EC - 3161 respectively. In this study, RJ - 2020 and EC - 3161 of sorghum genotypes exhibited drought tolerance and these genotypes could be used for application in breeding program.