Prevalence of Pseudomonas Aeruginosa in Hospitalised Patients in Kubwa General Hospital, Abuja, FCT

                                      ABSTRACT

The present study was to determine the prevalence of pseudomonas aeruginosa in patients using clinical samples (urine, skin lesion and pus) and environmental samples (sand, tap water, flowers). A total of thirty (30) samples were collected from patients, ten (10) each of urine, skin lesions and pus. And also three environmental samples (tap water, sand and flower) were collected. The samples obtained were cultured using standard microbiological methods and the isolates obtained were identified based on their colonial morphology, motility testing, gram staining reaction and biochemical tests. The antimicrobial sensitivity pattern of the isolates to ten (10) different antibiotics was determined. The results obtained showed that the micro organisms seen in the sample were staphylococcus spp, Escherichia spp, Klebsiella spp, proteus spp and Pseudomonas aeruginosa. The environment samples were negative for Pseudomonas aeruginosa but staphylococcus spp and Escherichia spp were seen. The percentage prevalence of the isolated organism from the environment samples was 50% in each. The prevalence (%) of p. aeruginosa isolated from clinical samples were 12.5%(urine), 31.25%(lesion)  and 15.38%(pus). The results were also determined according to sex for each sample. For male, urine (6.25%), skin lesion (18.75%) and pus (7.69%) also for female, urine (6.25%), skin lesions (12.5%) and pus (7.69%). The presence of Pseudomonas aeruginosa is of public health significance and these findings indicates the prevalence of Pseudomonas aeruginosa, thereby providing clues to prevention and treatments.

TABLE OF CONTENTS

Title Page                                                                                                   i

Certification                                                                                              ii

Dedication                                                                                                 iii

Acknowledgement                                                                                   iv

Abstract                                                                                                     v

Table of contents                                                                                      vi

CHAPTER ONE

1.0           Introduction                                                                                    1

1.1    Justification                                                                                     3

1.2    Aims and Objectives                                                                       4

CHAPTER TWO

LITERATURE REVIEW

2.1    Classification History of Pseudomonas                                         6

2.2    Pseudomonas group and their characteristics                             7

2.3    Epidemiology of Pseudomonas Aeruginosa                                 9

2.4    Cultural Characteristics                                                                  12

2.5    Identification of Pseudomonas Aeruginosa                                  12

2.6    Pathogenesis                                                                                   17

2.7    Signs and Symptoms                                                                      24

2.8    Laboratory Diagnosis                                                                      30

2.9    Other Method of Diagnosing Pseudomonas Aeruginosa            31

2.10  Treatment                                                                                        33

2.11  Prevention and Control                                                                  36

CHAPTER THREE

MATERIALS AND METHODS

3.1    Study Area                                                                                       38

3.2    Method                                                                                            39

3.3    Samples Collection and Processing                                               40

3.4    Viable Count                                                                                    43

3.5    Isolation and Identification                                                            43

3.5.1Biochemical Test                                                                             45

CHAPTER FOUR

Results                                                                                                       48

CHAPTER FIVE

5.1    Discussion                                                                                        65

5.2    Conclusion and Recommendation                                       68

References                                                                                       70

Appendix                                                                                                   80