Prey Alence Of Dengue Virus In Mosquitoes Caught In Oye Ekiti, Ekiti State, Nigeria

ABSTRACT

Dengue Virus is the most prevalent arthropods borne viral infection in tropical and

sub-tropical regions worldwide and they are transmitted by Aedes aegypti and Aedes

albopictus. This experiment was conducted with the aim of detecting and

establishing possible sylvatic transmission of Dengue Virus in mosquitoes caught in

Oye Ekiti. Mosquito life traps were used to trap 200 Aedes mosquitoes which were

then knocked down by the use of insecticides. RNA were extracted from the

mosquitoes and amplified using PCR machine. The amplified RNA were loaded into

gel and electrophoresis machine was used to test for the presence of Dengue Virus in the sample. In all the mosquitoes tested, no Dengue Virus was detected.