Seed Health Testing For Nine Cultivars of Sorghum bioclor L. in Gadarif Area

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This study was conducted in both laboratories of plant pathology and tissue culture in College of Agricultural Studies, Sudan University Science and Technology Nine cultivars of Sorghum bicolor collected from Gadarif area were tested for the presence of seed borne fungi. The cultivars were Mugudahmer, Wadahmed, Wadakar. Arfaagadamac, Hageen, Tetron, Dabar, Fatarita and Tabet. Seed health testing included dry inspection in which the seeds were divided into healthy, broken and infected. Seeds were germinated on moistened medical cotton in sterilized plastic petridishes and as well seeds were plated on moistened filter paper

and incubated at 25o C . Seven fungi were isulated,purified and identified . These were; Drechslera spicifer, Macrophomina phaseolina, Fusarum oxysporum, Penicillum digitatum, Spergillus flavus,Aspergillus niger,

Rhizopus nigricans. Pathogenicity test was carried out for four fungi these were Drechslera spicifer, Macrophomina phaseolina, Fusarum oxysporum, Penicillum digitatum. The results revealed that the symptoms produced from artificial inculcations were seed rot bre-and post- emergence damping off as well leaf spots. The fungi Macrophomina phaseolina, recorded the highest incidence 26.6% in cultivars Wad ahmed and the least incidence 6.6% in cultivars Hageen. Wad ahmed and Arfaagamac the highest bos- emergence damping off was 40% in cultivar Daber and the least incidence 20% in cultivars Wad

ahmed. Wad akar and Arfaa gadamac. For symptoms development the highest Arfaa gadamac 26.6% and the least incidence was 6.6% in cultivars Daber and Fatarita. For Drechslera spicifer, the highest incidence for seed rot and pre emergence damping off was 33.3% for Wad akar arfaaga gadmac and the least incidence in cultivars Daber For symptoms development the highest incidence was 20% in Wad ahmed, Arfaagadamac Fatarata, Tabet and the least incidence was 6.6% in cultivar Daber. For Fusarum oxysporum the highest pre emergence damping off was33.3% in cultivars Tabet, Tetron, Hageen, Wadahmed and Arfaa gadamac And the least was 13.3% in cultivar Daber The highest post emergence damping off was 40 in Daber and the least incdence13.3% in cultivars Mugud ahmer, Hageenand Daber. For symptoms development the highest in cultivars

Wad Ahmed 33.3% and the least mdence was 13.3% in cultivars Daber Hageen and Nugud ahmer.

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Seed Health Testing For Nine Cultivars of Sorghum bioclor L. in Gadarif Area

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