Soybean (Glycine Max) Response To Rhizobia Inoculation As Influenced By Soil Nitrogen Levels

ABSTRACT

Inoculation of soybean (Glycine max) is an efficient and convenient way of introducing rhizobia to soil and subsequently the rhizosphere of the crop. However, its full potential in sub-Saharan Africa is yet to be realized due to effects of varying soil limiting conditions. Critical levels of nitrogen (N) below and above which response is not guaranteed are unknown. The objective of the study was to determine the critical range of N outside which response to inoculation is hindered. Below the lower limit, an adequate starter N would be required, while above the upper limit, inoculation would not be effective. A greenhouse experiment was set up with sixty soils with a varying range of total N (%) and organic carbon ≤ 2.1 %. The experiment setup was a Complete Randomized Design (CRD) with and without inoculation replicated three times. A second greenhouse experiment with two soils selected based on the first experiment was used (with 0.06 % N and 0.08 % N). A complete randomized design was used with three replicates and five rates of an organic N source (i.e. 0, 2.5, 5, 7.5 and 10 t Phymyx ha-1 ) for each soil N level, with and without inoculation to assess whether the organic amendment could improve the response to inoculation within the adequate range of N. A field experiment was set to validate the 2 ndgreenhouse experiment results under field conditions. A split plot arrangement in a randomized complete block design (RCBD) design full factorial with three replicates for the two sites was set up. The main plot as Sympal (contains phosphorus, potassium, Sulphur, calcium, magnesium, and zinc) applied at a rate of 30 kg P ha-1 and the sub-plots had Phymyx at 5 levels with and without inoculation. Soybean variety TGx1740-2F was inoculated with Bradyrhizobium japonicum strain 532c. In the first greenhouse trial, it was demonstrated that soybean response to inoculation was significantly affected by soil fertility based on nodule fresh weight and shoot biomass, but on average the two parameters were improved by inoculation with Legumefix (p