ABSTRACT
The effect of concentration of hydrochloric acid on hydrolysis of cellulose (saw-dust) to glucose was studied on this research project and the steps obtained to achieve this project involved treatment of saw-dust (cellulose) with different concentrations of the acid at constant temperature of 80° (350k) for 30mins. This was followed by glucose analysis, some analysis or experiments were done on acid hydrolysis in order to study the effect of (HCL) acid on the hydrolysis of cellulose to glucose. The process used in this hydrolysis was acid hydrolysis in which HCL acid was used at constant temperature of 80oC and the saw-dust used [was obtained by grinding wood with saw] was weighed and mixed with water . Secondly, during this analysis/experiment, it was observed that hydrochloric acid hydrolyzed well from the readings gotten from each result that was carried out during the analysis. Then lastly, glucose analysis was carried out to determine the absorbance and glucose concentration. It was noticed that the best concentration of HCL acid during hydrolysis yields glucose concentration of 0.127g or 1.270%.
TABLE OF CONTENT
CHAPTER ONE
1.1Introduction1
1.2Definition of Terms3
1.3Statement of the Problem4
1.4Scope and of Study Limitations5
1.5Objectives6
CHAPTER TWO
2.1Literature Review8
2.2History9
2.3Products10
2.4Cellulose Source and Energy Store of Crops. .............. 12
2.5Structure and Properties14
2.6Biosynthesis18
2.7Breakdown (Cellucolysis). ............................................ 22
2.8Hemicellulose24
2.9Derivatives24
2.10 Functionality29
2.11 Occurrences30
2.12 Classification of Cellulose31
2.12.1Cellulose Acetate. ...................................................... 32
2.12.2Cellulose Acetate Butyrate. ........................................ 32
2.12.3Cellulose Nitrate. ...................................................... 33
2 12 .4 Methyl Cellulose.-34
2.12.5Ethyl Cellulose. ......................................................... 34
2.12.6Carboxy Methyl Cellulose. ........................................ 35
2.13Regenerated Cellulose. ................................................ 36
2.14Uses of Sugar. ........................................................... 38
2.15Sugar in Foods40
2.16Functions of Sugar.40
2.17Natural Polymers of Sugar. ........................................ 41
2.18Types of Sugar. .......................................................... 42
CHAPTER THREE
3.1Materials and Equipment46
3.1.1 Materials:46
3.1.2 Apparatus:46
3.1.3 Reagent47
3.2Acid Hydrolysis (Hcl)48
3.2.1 Procedure:48
3.2.2Glucose Analysis Colorimetric (Using Benedict’s) Method. 49
3.2.3 Procedure:50
The Glucose Concentration (Hcl) Was Determined Using
Beer Lambat Law.50
CHAPTER FOUR
Results and Discussion
4.1Results52
4.2Discussion53
CHAPTER FIVE
5.0Conclusion and Recommendation55
5.1 Conclusion55
5.2 Recommendation56
References58
Appendix A60
Appendix B61
LIST OF TABLES
Table 4.1: variation of absorbance with the standard glucose concentration
Table 4.1.2: variation of absorbance with hydrochloric acid
LIST OF FIGURE
Figure 4.1: graph of absorbance of the acid against the glucose concentration.
Figure 4.2: Cellulose
Figure 4.3: Strand of Cellulose
Figure 4.4: Triple strand of Cellulose.
Introduction
Cellulose is the name given to a long chain of atoms consisting of carbon, hydrogen and oxygen arranged in a particular manner it is a naturally occurring polymeric material containing thousands of glucose-like rings each of which contain three alcoholic OH groups. Its general each of which contain three alcoholic OH groups. Its general formula is represented as (C6H1005)n. the oh-groups present in cellulose can be esterifies or etherified, the most important cellulose derivatives are the esters.
Cellulose is found in nature in almost all forms of plant life’s, and especially in cotton and wood. A cellulose molecule is made up of large number of glucose units linked together by oxygen atom. Each glucose unit contains three(3) hydroxyl groups, the hydroxyl groups present at carbon-6 is primary, while two other hydroxyl are secondary. Cellulose is the most abundant organic chemical on earth more than 50% of the carbon is plants occurs in the cellulose of stems and leave wood is largely cellulose, and cotton is more than 90% cellulose. It is a major constituent of plant cell walls that provides strength and rigidity and presents the swelling of the cell and rupture of the palms membrane that might result when osmotic conditions favor water entry into the cell. Cellulose is a fibrous, ought, water-insoluble substances, it can be seen in cell walls of plants, particularly in stalks, stems, trunks and all woody portions of the plant.
Cellulose is polymorphic, i.e there are number of different crystalline forms that reflect the history of the molecule. It is almost impossible to describe cellulose chemistry and biochemistry without referring to those different forms. Cellulose are gotten from cellulose, cellulose is also found in protozoa in the gut of insects such as termites. Very strong acids can also degrade cellulose, the human digestive system has little effect on cellulose. The world cellulose means β-1, 4- D glucan, regardless of source because of the importance of cellulose and difficulty in unraveling its secrets regarding structure, biosynthesis, chemistry, and other aspects, several societies are dedicated to cellulose, lignin, and related molecues.