The Prevalence Of Brucella Abortus And Mycotoxin Contamination Of Animal Feeds And Implications On Milk Safety In Rural And Peri-Urban Small-Holder Dairy Systems In Nakuru County, Kenya

ABSTRACT

Mycotoxin and Brucella abortus contamination of milk along the dairy value chain is of public health concern and contributes to food insecurity. Animal feeds that are contaminated with mycotoxin causing fungi pass the contaminationtion to the milk. Inhygienic handling of milk can lead to Brucella contamination of milk. This study aimed at determining the on-farm risk factors associated with Mycotoxins and B. abortus occurrence and their prevalence on smallholder farms at rural and peri-urban dairy sub-value chains in Nakuru County. A crosssectional survey using semi-structured questionnaires was administered randomly to 280 farmers and raw milk market players. Alongside, milk samples (317), blood for serum ((n=245) and animal feed (74) samples were collected. Feed samples were analyzed for Aflatoxin (AFs) concentration and Deoxynivalenol (DON) and milk samples for Aflatoxin M1 (AFM1) using commercial Enzyme Linked Immune Sorbent Assay (ELISA). Serum samples were first screened for antibodies against B. abortus using milk ring test (MRT) and Rose Bengal Plate test (RBPT). Positive reactors were further confirmed by Complementary Fixation Test (CFT) and competitive enzyme linked immunoassay (c-ELISA) test. Data was analyzed using SPSS version 20 for qualitative data and SAS version 9 for the quantitative data and Means were compared using least significant differences (LSD) at 95% confidence level. The study identified three risk factors for mycotoxin contamination of animal feeds at the farm level; 1) the type of feeds, 2) condition of feed and 3) storage method. Type and condition of animal feeds were found to be significant P