Isolation, Identification and Antimicrobial Susceptibility Test for Various Bacteria Present in Blood

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ABSTRACT

Bacteremia is one of the most significant public health concerns and is a challenge in critical care of a patient. Early diagnosis of bacteremia could be vital step in estimating appropriate treatment as well as beginning of it. The current study aims to discover the pervasiveness of different bacterial species responsible for causing bacteremia around the population of various gender and age group and enabling the doctors for choosing the verifiable antimicrobials through antimicrobial susceptibility patterns. The diagnosis was achieved by isolation and identification of the bacteria, causing infection. 1105 samples of blood were collected from suspected patients of different gender as well as age groups. Samples were processed according to the standard microbiological protocol during the period from 1 January 21 to 31 March 21. Out of total screened blood samples, only 110 cultures were found to be positive, from which 59 Gram-negative bacterial isolates and 51 Gram-positive bacterial isolates were identified, and their antimicrobial susceptibility testing was performed through fully automated VITEK2 Compact. Salmonella typhi and Salmonella paratyphi A were the most predominant Gram-negative bacteria isolated. Staphylococcus epidermis and Staphylococcus hominis were most predominant Gram-positive bacteria isolated. Few other Staphylococcus, Enterococcus and E.coli were also identified in the remaining isolates. The study reflected that males were more suspected to bacteremia as compared to females and age group

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Isolation, Identification and Antimicrobial Susceptibility Test for Various Bacteria Present in Blood

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