ABSTRACT
There is a growing industrial demand for peptones of non-animal origin owing to legislations as
well as outbreak of animal-associated diseases. Microbiological growth media developed from
legumes as sources of protein hydrolysates are limited. It is, therefore, the aim of this study, to
produce comparatively standard protein hydrolysates from Prosopis africanaseed that can be
used as peptone for microbial culture in place of animal peptone.
Prosopis africana seeds (2.5kg) were purchased from a local market in Mbu-Akpoti, Enugu
State of Nigeria. Stones and bad seeds were sorted out. The selected seeds were washed using
distilled water and then boiled until the seed coats became soft. The softened seed coats were
manually peeled off to reveal the cotyledons, which were collected, washed, dried and powdered.
The powdered cotyledon was subjected to hydrolysis using the enzyme papain or hydrochloric
acid. Metal ions, vitamins as well as proximate compositions of the hydrolysates were
determined using standard methods. Amino acid analysis was done using HPLC. Growth
patterns of Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli,
Candida albicans and Aspergillus niger were monitored in the media formulated using the seed
hydrolysates. Peptone in commercially available MacConkey, Mannitol-salt and Sabouraud
dextrose media was substituted with the seed hydrolysates and these were used as selective or
differential media for specific organisms.
In assessing the above parameters, comparism between commercially available peptone and the
hydrolysates from Prosopis africana seed were made.
The results of the analyses revealed that papain hydrolysed Prosopis africana seed (PHPs)
ranked highest in terms of microbial growth support when compared to AHPs. PHPs and AHPs
had moisture contents of 32.7and 83.3% respectively as against 3.93% in CP. The ash values
were 3.05 and 0.15% for PHPs and AHPs respectively whereas CP had 4.08%. Crude proteins of
39.69 and 12.78% were also observed in PHPs and AHPs respectively. The hydrolysates were
found to be rich in vitamins A, B, C and E and also in metal ions. Serine, threonine and lysine
were the most dominant amino acids in the seed hydrolysates. Escherichia coli, Staphylococcus
aureusand Candida albicans all thrived well in their respective selective media made with the
hydrolysates, and this compared favourably well with commercially available peptone.
Production and use of Prosopis africana seed hydrolysate as source of peptone will help to bring
down the cost of peptones which are currently being imported and sold at very high rates in
Nigeria.
IKECHUKWU, O (2021). Use Of Prosopis Africana Seed Hydrolysate As Peptone Source For Microbial Growth Media. Afribary. Retrieved from https://tracking.afribary.com/works/use-of-prosopis-africana-seed-hydrolysate-as-peptone-source-for-microbial-growth-media
IKECHUKWU, ONAH "Use Of Prosopis Africana Seed Hydrolysate As Peptone Source For Microbial Growth Media" Afribary. Afribary, 13 May. 2021, https://tracking.afribary.com/works/use-of-prosopis-africana-seed-hydrolysate-as-peptone-source-for-microbial-growth-media. Accessed 22 Dec. 2024.
IKECHUKWU, ONAH . "Use Of Prosopis Africana Seed Hydrolysate As Peptone Source For Microbial Growth Media". Afribary, Afribary, 13 May. 2021. Web. 22 Dec. 2024. < https://tracking.afribary.com/works/use-of-prosopis-africana-seed-hydrolysate-as-peptone-source-for-microbial-growth-media >.
IKECHUKWU, ONAH . "Use Of Prosopis Africana Seed Hydrolysate As Peptone Source For Microbial Growth Media" Afribary (2021). Accessed December 22, 2024. https://tracking.afribary.com/works/use-of-prosopis-africana-seed-hydrolysate-as-peptone-source-for-microbial-growth-media