Abstract The effect of Shea butter rich diet on gentamicin induced nephrotoxicity in rats was determined. Thirty (30) male and female albino rats grouped into 6 (normal, negative, positive, 10%, 20% and 30% Shea butter rich diet groups respectively) of 5 rats each were used for the study. Phytochemical analysis was carried out on the Shea butter used for this study which revealed the presence of saponin, tannin, alkaloid, terpenoid, flavonoid, and reducing sugar, while coumarin was absent. ...