The in vivo activities of inulin and ascorbic acid were evaluated experimentally via using 450 Nile tilapia (Oreochromis niloticus) that were distributed into 3 equal groups (each of three replicates). Fish of the first group served as a control and received a balanced diet free from inulin and vitamin C. The second fed on balanced diet supplemented with inulin (5 g kg1), whereas, the third one received a balanced diet supplemented with vitamin C (500 mg kg1). The survival and growth perf...
The purpose of this study was to examine the effect of some immunostimulants and probiotics on the survival rate, final weight, and disease resistance of overwintered tilapia fry. There were five treatments: T1 (control) fed a balanced diet (35% protein) without additives. Treatments 2 to 5 were fed diets supplemented with 4% garlic, 4 g/kg Echinacea, 4 g/kg Organic Green or 4 g/kg Vet-Yeast, respectively. Growth and resistance to disease challenge with Aeromonas hydrophila and Pseudom...
This study was conducted in order to evaluate the potential bene¢t of Bacillus pumilus and a commercial product (‘Organic Green’TM) as a probiotic in the culture of the tilapia nilotica. Two doses of B. pumilus (106 and 1012 g1 diet fed) and Organic GreenTM (1 and 2 g kg1 diet fed) were used as feed additives and administered for periods of 1 (groups 2, 3, 4 and 5 respectively) and 2 (groups 6, 7, 8 and 9 respectively) months; group 1 served as a control. Each group consisted of...
A total of one thousand Oreochromis niloticus held in earthen ponds of the WorldFish Center were used in this study. Forty of these were randomly collected from the ponds and subjected to bacteriological and pathological examination where a variety of 80 bacteria were isolated. Among those 15 bacterial isolates were examined for their probiotic activity through studies performed in vitro and in vivo. Aeromonas hydrophila, Citrobacter freundii, Pseudomonas species and two Gram-positive isola...
Three dosage levels of black seed, garlic and commercial Biogen were administered for three months in summer and six months in winter to test their stimulation effect on growth, survival and response to challenge infection in Oreochromis niloticus. At the end of summer, no significant difference was observed in the growth performance of O. niloticus with most treatments, but the level of non-specific mortalities was reduced in all treatment groups compared to the control group. Significant...
The probiotic activity of two bacteria (Bacillus subtilis and Lactobacillus acidophilus) was evaluated by its effect on the immune response of Nile tilapia (Oreochromis niloticus), beside its protective effect against challenge infections. Furthermore, their in-vitro inhibitory activity was evaluated. The in-vitro antimicrobial assay showed that Bacillus subtilis and Lactobacillus acidophilus inhibited the growth of A. hydrophila. The B. subtilis inhibited the development of P. fluorescen...
To evaluate the effect of some immunostimulants on growth performance parameters, survival rate and diseases resistance of Nile tilapia fry reared under different stocking densities, a total number of 2250 Oreochromis niloticus fries (0.3 ±0.05 g) were stocked at (2, 4, and 6 fry/m3) and provided with dietary supplementation of (Garlic 40 g/kg, purpurea extract 4 g/kg, Organic GreenTM 4 g/kg and Vet-YeastTM 4 g/kg of the diet) for a five-month period. The obtained results indicated that, sto...
This work aimed to investigate the role of some immunostimulants and probiotics in improving the response of overwintered tilapia to Aeromonas hydrophila vaccine. In this study, 15000 Nile tilapia fry (Orechromis niloticus) were collected and divided into five groups. Group 1 was the control, groups 2 to 5 were fed diet supplemented with garlic, Echinacea, Organic GreenTM and Vet-YeastTM respectively for 5 months. Vaccination with A. hydrophila bacteria was done by the end of the feeding e...
Our aim was to detect potential pathogens in diseased tilapia that might account for the high summer mortalities observed in Egyptian fish farms. To this end, diseased fish were examined from eight farms in different areas of the Nile delta. Here, we report the detection of Aeromonas species and, for the first time, the presence of TiLV in Egyptian tilapia aquaculture.
Egyptian fish farms have faced unexplained mortality of tilapia during the summer months in recent years. Epidemiological surveys indicated that 37% of fish farmswere affected in 2015with an averagemortality rate of 9.2% and a potential economic impact of around US$ 100 million. Despite a number of researchers and organizations investigating potential causes results so far have been inconclusive. Meanwhile recent reports emerged of the presence of a new orthomyxovirus, Tilapia Lake Virus ...
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