Natural & Applied Sciences

Research Papers/Topics Natural & Applied Sciences

ABC transporter genes ABC-C6 and ABC-G33 alter plant-microbeparasite interactions in the rhizosphere

Abstract: Plants are master regulators of rhizosphere ecology, secreting a complex mixture of compounds into the soil, collectively termed plant root exudate. Root exudate composition is highly dynamic and functional, mediating economically important interactions between plants and a wide range of soil organisms. Currently we know very little about the molecular basis of root exudate composition, which is a key hurdle to functional exploitation of root exudates for crop improvement. Root exp...

Complete Reference Genome Assembly for Commensalibacter sp. Strain AMU001, an Acetic Acid Bacterium Isolated from the Gut of Honey Bees

Abstract: We report here the genome sequence of a Commensalibacter sp. strain (AMU001) isolated from honey bees (Apis mellifera) from Seychelles. By combining long- and short-read sequencing technologies, we produced the first complete reference genome assembly for the Commensalibacter genus. We anticipate that this will aid future comparative and functional genomic studies.

Spatial Distribution of Cocoon Nests and Egg Clusters of the Silkmoth Anaphe panda (Lepidoptera: Thaumetopoeidae) and its Host Plant Bridelia micrantha (Euphorbiaceae) in the Kakamega Forest

Abstract: A study on the spatial distribution of the silkmoth Anaphe panda (Boisduval) cocoon nests, egg clusters and the host plant Bridelia micrantha (Hochst) Baill. was conducted in two different habitats of the Kakamega Forest of western Kenya: Ikuywa (indigenous forest) and Isecheno (mixed indigenous forest). The mean densities of cocoon nests, egg clusters and B. micrantha were significantly different in the two blocks and were not semi-randomly distributed in the two habitats. The hos...

Semi-captive Rearing of the African wild Silkmoth Gonometa postica (Lepidoptera: Lasiocampidae) on an Indigenous and a Non-indigenous Host Plant in Kenya

Abstract: The life cycle of the African wild silkmoth Gonometa postica Walker was studied between September 2000 and September 2001 in the Uasin Gishu District of western Kenya with Acacia mearnsii deWild and Acacia hockii deWild as host plants. The initial population of G. postica was set up from healthy live pupae in cocoons collected from host plants in the study area. Oviposition was carried out in net sleeves and small plastic cages. Moth emergence and oviposition were bimodal, occurrin...

Bacterial diversity in the intestinal tract of the funguscultivating termite Macrotermes michaelseni (Sjöstedt)

Abstract: Microorganisms in the intestinal tracts of termites play a crucial role in the nutritional physiology of termites. The bacterial diversity in the fungus-cultivating Macrotermes michaelseni was examined using both molecular and culture dependent methods. Total DNA was extracted from the gut of the termite and 16S rRNA genes were amplified using bacterial specific primers. Representatives from forty-one (41) RFLP patterns from a total of one hundred and two (102) clones were sequence...

A Review of African Blastobasinae (Lepidoptera: Gelechioidea: Coleophoridae), with New Taxa Reared from Native Fruits in Kenya

Abstract: Adamski, David, Robert S. Copeland, Scott E. Miller, Paul D. N. Hebert, Karolyn Darrow, and Quentin Luke. A Review of African Blastobasinae (Lepidoptera: Gelechioidea: Coleophoridae), with New Taxa Reared from Native Fruits in Kenya. Smithsonian Contributions to Zoology, number 630, vi + 68 pages, 62 figures, 13 maps, 2 tables, 2010. — Twenty-five species of African Blastobasinae (Lepidoptera: Coleophoridae) are reviewed; 12 species are redescribed, and 13 species are described a...

Evolutionary Genomics at the Human–Environment Interface in Africa

Abstract: We report on the first meeting of SMBE in Africa. SMBE Malawi was initiated to bring together African and internationalresearchers who use genetics or genomics to study natural systems impacted by human activities. The goals of thisconference were 1) to reach a world-class standard of science with a large number of contributions from Africa, 2) toinitiate exchange between African and international researchers, and 3) to identify challenges and opportunities forevolutionary genomics...

The relative contributions of host density and genetic diversity on prevalence of a multi-host parasite in bumblebees

Abstract: The role of population and demographic factors in influencing the transmission and establishment parasites infect-ing multiple hosts is poorly understood. We assessed the effects of these factors on parasite prevalence in a model system – the intestinal protozoan Crithidia bombi (Trypanosomatidae) infecting a range of bumblebee species (Bombus spp.). We used microsatellite markers and sibship reconstruction to infer genetic diversity and the density of host populations to infer t...

Effect of alternative rearing substrates and temperature on growth and development of the cricket Modicogryllus conspersus (Schaum)

Abstract: The increasing demand for animal protein in sub-Saharan Africa has led to insects being considered an alternative protein source for food and feed. In Uganda, chicken mash, containing fish meal as the protein source is commonly used to rear crickets. However, fishmeal is an expensive protein source that is also human food. This study therefore aimed at establishing an alternative preferred (consumed in the highest quantity) plant leaf diet for raising the Modicogryllus conspersus c...

The period of insect research in the tropics: a bibliometric analysis

Abstract: The International Journal of Tropical Insect Science (Int J Trop Insect Sci) is a peer-reviewed journal established in 1980 to promote insect science mainly in the tropics. This study aimed to provide a Bibliometric overview of Int J Trop Insect Sci publications and citations between 2012 and 2020, ending September 2020. A sample of 488 documents extracted from the Web of Science (WoS) was analysed using widely used bioclimatic indicators. The articles were written by 1726 authors....

Characterization of honeys produced by sympatric species of Afrotropical stingless bees (Hymenoptera, Meliponini)

Abstract: We investigated the effect of bee species identity and harvesting methods on the chemical composition and antiradical activity of 53 honey samples, produced by six stingless bee species in western Kenya (Kakamega forest). Our results illustrate that none of the assayed parameters significantly varied between the honey samples harvested by “punching holes” (n = 25) and “squeezing” (n = 28) methods. By contrast, species identity drove significant differences in the assayed pa...

Diversity and phylogenetic analysis of endosymbionts fromTrioza erytreae(Del Guercio) and its parasitoids in Kenya

Abstract: The African citrus triozid (ACT), Trioza erytreae (Del Guercio), is the primary vector of Candidatus Liberibacter africanus (CLaf), the causative agent of Africa citrus greening disease (ACGD). This study evaluates the diversity of ACT parasitoids and further characterizes endosymbionts associated with both T. er y treae and its parasitoids that could be used as biological control agents of T. er y treae and management of ACGD. Mitochondrial cytochrome oxidase I gene was used to re...

Genetic Diversity, Distribution, and Genomic Characterization of Antibiotic Resistance and Virulence of Clinical Pseudomonas aeruginosa Strains in Kenya

Abstract: Pseudomonas aeruginosa is a leading cause of nosocomial infections worldwide. It can produce a range of debilitating infections, have a propensity for developing antimicrobial resistance, and present with a variety of potent virulence factors. This study investigated the sequence types (ST), phenotypic antimicrobial susceptibility profiles, and resistance and virulence genes among clinical isolates from urinary tract and skin and soft tissue infections. Fifty-six P. aeruginosa clin...

Prediction of breeding regions for the desert locust Schistocerca gregaria in East Africa.

Abstract: Desert locust outbreak in East Africa is threatening livelihoods, food security, environment, and economic development in the region. The current magnitude of the desert locust invasion in East Africa is unprecedented and has not been witnessed for more than 70 years. Identifying the potential breeding sites of the pest is essential to carry out cost-effective and timely preventive measures before it inflicts significant damage. We accessed 9,134 desert locust occurrence records an...

Protein Content and Amino Acid Profiles of Selected Edible Insect Species from the Democratic Republic of Congo Relevant for Transboundary Trade across Africa

Abstract: This study analyzed the protein content of ten edible insect species (using the Dumas method), then focused on the amino acid (AA) profiles of the six major commercially relevant species using HPLC (high-pressure (or performance) liquid chromatography). The protein contents varied significantly from 46.1% to 52.9% (dry matter); the Orthoptera representative yielding both the highest protein content and the highest values in three essential amino acids (EAAs). Regarding Lepidoptera ...


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