________________________________(Received: 08:03:2017; Accepted: 05:04:2017) _______________________ Abstract Lipase from Aspergillus flavus was purified in a single step purification using MnFeO4 magnetic nano particles to achieve a 20.53- fold purification with specific activity of 11.29 U/mg and a 59% recovery yield. SDS-PAGE of lipase showed a single pure band with corresponding molecular weight of 35 kDa. The optimal temperature and pH for the enzyme activity were 45°C and 7.0 respe...
ABSTRACT Bio-fuels are prominent in current discussions both as a solution to problems and as a creator of problems. They have promise as a substitute for fossil fuels, particularly petroleum as the raw material for transportation fuel. But bio-fuels also have pitfalls, especially when produced at a scale sufficient to replace a significant proportion of the world's use of petroleum. This controversial topic has attracted great political, economic and social attention because of its touching...
ANTIBIOTIC SENSITIVITY TESTING ON EGGS FROM LAYERS TREATED WITH ANTIBIOTIC A RESEARCH PROJECT ABSTRACT Antibiotic sensitivity testing is the susceptibility of bacteria to antibiotics, it is carried out to determine which antibiotic will be most successful in treating a bacterial infections. The total mean colony count of the bacterial isolates in the two set of egg sample carried out showed the mean total bacterial count as 0.33x102 – 3.6 x 102 cfu/ml. While the antibiot...
ANTIBIOGRAM OF BACTERIAL ISOLATED FROM SEALED AND UNSEALED DRUGS A RESEARCH PROJECT ABSTRACT The study on the isolation and antibiogram of bacterial isolated form sealed and unsealed drugs revealed total Bacteria count ranging form 1.2 X 107 to 11.5 X108cfu/g for unsealed and 1.5 X107 to 9.2 x 107cfu/g for sealed. The following bacterial isolates were identified these include Bacillus sp, klebsiellssp, staphylococcus sp Escherichia sp, Lactrbacillussp, proteussp and Citrobacter...
This purpose of this study is to determine the Inhibitory zone diameter (Izd), phytochemical screening, elemental composition and proximate analysis of crude Cleistopholis patens leaf, bark, aqueous and ethanol extracts on selected pathogenic isolates. The leaf and bark of Cleistopholis patens plants were obtained from a location in the southwestern part of Nigeria, in the tropical rainforest of Ikare Akoko, Ondo state and Ile Ife, Osun state, Nigeria. The six (6) bacterial isolates include E...
Abstract Background Newcastle disease is a highly contagious disease of birds caused by Paramyxovirus. Birds mostly affected by Paramyxovirus virus includes; chickens, turkeys, geese, ducks and guinea fowls. Newcastle disease however, remains a worldwide problem. There are three major strains of Newcastle disease virus and they are velogenic, mesogenic and lentogenic strains respectively. These viral strains reflects the severity of the disease pathotypes. In the medical laboratory, the...
ABSTRACT Panteka stream is a flowing stream polluted with waste from the activities of mechanics. Water samples collected at different sampling points of the stream were analysed in order to determine the level of heavy metal contamination and bacteria diversity with the view to elucidating the bioremediation potentials of the bacteria isolates. Four bacteria species with high tolerance to lead, zinc, iron, nickel and cadmium were identified by morphological and biochemical analysis as ...
A human or animal with an infection has another organism inside them which gets its sustenance (nourishment) from that person, it colonizes that person and reproduces inside them. The human with that organism (germ) inside is called the host, while the germ or pathogen is referred to as a parasitic organism. Another name for an organism that causes infection is an infectious agent. It is only an infection if the colonization harms the host i.e parasitic relationship. It feeds and multiply ...
Bacteria are group of microorganisms all of which lack a distinct nuclear membrane (and hence are considered more primitive than animal and plant cells) and of most which have a cell wall of unique composition. Most bacteria are unicellular; the cells maybe spherical (coccu), rod-shaped (bacillus), spiral (spirillum), comma-shaped (vibrio) or corkscrew-shaped (spierocheate). Generally, they range in size between 0.5 and 5um. (Elizabeth and Martin, 2003). Food is any substance that people or ...
The search for potent antimicrobial agent is unending. The use of mouthwashes to improve oral hygiene is being favoured for its presumed antimicrobial activity. This study therefore evaluated the antibacterial activity of common mouthwashes against relevant clinical isolates. Five mouthwash samples (coded; LIS; COL; TOT; FLU; BRE) bought from major super markets in Ondo State, Nigeria, were examined for antimicrobial activity using agar well diffusion method. The kinetic of activity and M...
Artificially inoculated fruit juice is a very viable profit making sole business in Nigeria, the product is locally available in all market in different parts of the country, especially in Ondo state, using Ikare and Iwaro Akoko market, as the focal point of this present study. Fifty (50) samples of the inoculated fruit juice were purchase from the market in Iwaro and Ikare Akoko, Ondo State. The test organisms used for this research work are Staphylococcus aureus (ATCC) 25923, Salmonella spp...
The drying efficacy and product quality of onion and pepper dried in the oven and sun were compared. The drying process took 1-4 days to accomplish. The proximate analysis, bacterial load and identification of microorganisms, sugar fermentation, catalyses test and the bacteria count were determined. The mean moisture content of the product at the end of the drying was 6.4% (sun) and 4.5% (oven). The oven dried product were significantly (P
The Aqueous, ethanol, hexane and ethyl acetate extracts of the leaves, bark and roots of Acacia albida, Anchomanes difformis, Boscia senegalensis, Bridelia ferruginea, Ficus ingens, Indigofera arrecta and Moringa oleifera were tested for possible source of antibacterial activities against selected clinical organisms found in the pediatrics hospital in Ondo and Ekiti State, Nigeria. The selected clinical organisms used for this research work are Salmonella typhi, Staphylococcus aureus, Klebsie...
Morinda lucida is a tropical West African evergreen shrub or small to medium sized rainforest tree, having brown colored bark, white flowers and yellowish ellipsoid seeds. It is widely used in traditional medicine systems of different countries. This study was designed to carry out the preliminary phytochemical screening and evaluation antioxidant and proximate properties of ethanol extracts prepared from the bark, leaves and root of M. lucida . It revealed the presence of saponin, tannins, a...
Erythrina senegalensis DC. (Coral tree) is commonly grown in West Africa as an ornamental plant and one of the oldest known African medicinal plants. The purpose of this research is to determine the antimicrobial potency, phytochemical activities, nutrients and elemental composition of the leaf and bark of Erythrina senegalensis. The organisms used for the study are Escherichia coli, staphylococcus aureus, Candida albicans and Klebsiella pneumonia. The leaf and bark were extracted using one e...