Applied Microbiology Research Papers/Topics

Phytochemical Composition and Antimicrobial Activity of Daniella oliveri Extracts

Daniella oliveri (Caesalpiniacea) is a plant found in the Amazon region and other parts of South America and Africa. The tree may reach a height of 100 feet and trunk diameter of 4 feet. Daniella oliveri (Rolfe) Fabiaceae is commonly known as Ilorin balsam (eepo-iya) or Copaihu africana . It is an indigenous African tree, found extensively in Benin, Cameroon, Gambia and Nigeria. The basic objective of this project is to determine the antimicrobial activity and phytochemical composition of Dan...

Evaluation of Antibacterial Potency of Crude Ethyl Acetate and Ethanol Extracts of Morinda lucida

The plant Morinda lucida falls under the family Rubiaceae known to have wide usage in traditional medicine. Morinda lucida is a tropical West African tree of medium-size about 18–25 m tall, the bark is grey to brown in colour, flowers are white in colour, the fruit is a drupe, seed is ellipsoid, yellowish and soft. The purpose of this work is to determine the antimicrobial properties of      Morinda lucida against Mycobacterium species, a very virulent and infectious organism, isolated ...

Elemental Composition, Evaluation of Anti-nutrients, and Antioxidant Potentials of Morinda lucida

Morinda lucida is a tropical West African tree of medium-size, having brown colored bark, white flowers and yellowish ellipsoid seeds. It is widely used in traditional medicine systems of different countries. The preliminary phytochemical screening of different extracts prepared from bark, leaves and seeds of M. lucida revealed the presence of Saponin, Tannins, Alkaloids, Flavonoids, and Cardiac glucosides. Furthermore, it also reveals presence of Minerals like Sodium, Potassium, Calcium, Iro...

Use of the Biomerieux id 32c Fungal Identification System for Fungal Isolated from Mother and Child Hospital Dump Site in Akure, Ondo State Nigeria

The purpose of this research work is to isolate and identify Using ID 32C API kit and to determine the antifungal sensitivity profile of fungi isolated from the mother and child dump site Akure, Ondo state, Nigeria.   Ten isolates (Yeast, Aspergiliusflavus, Aspergiliusniger, Fusariumsolani, Candida albucan, Candida kruise, Phytoptera, Rhizopusstolonifer, Trichodermhorizianum,Fusariumvortezelium) were used for this research work.Potato Dextrose Agar was used to isolate and prepared for enume...

Bio-prospective Screening of Antibacterial and Phytochemical Activity of Caesalpinia pulcherrima.Linn (Pride of Barbados) on Selected Clinical Isolates

This work was carried out to evaluate the antibacterial and Phytochemical activities of Caesalpiniapulcherrima  leaf and stem bark on some clinical isolates. The plant parts were collected from St. Benedict Catholic Church premises, Owena Barracks, Akure, Ondo State. Crude ethyl acetate extracts of Caesalpinia pulcherrima leaves and stem bark was assayed for its antibacterial activity against Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa, Bacillus subtilis, Escheric...

Prospects of Essential Oils in Drug Discovery

Abstract Essential oils (EO), according to the International Standard Organization (ISO)  defines essential oil as the product obtained from parts of plants by distillation with water steam and the product obtained by expression from pericarp of medicinal plant. The rapid growth and increase of the knowledge of identification, extraction, purification and characterization of essential oils in most local plant species is fast becoming an important aspect of the drug production process. ...

Evaluation of Antimicrobial Activity, Synergistic Efficacy, Qualitative and Quantitative Phytochemical Determination of Alstonia Boonei Leaf and Stem Bark on Selected Clinical Isolates

Abstract This study evaluated the antimicrobial activity, of Alstonia boonei leaf, stem bark and the synergistic efficacy of A. boonei leaf plus stem bark on seven bacteria (Gram positive and Gram negative bacteria) which are strains of Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Proteus viridans, Salmonella typhi and Klebsiella pneumoniae, and three fungal isolates which are Candida albicans, Aspergillus flavus and Trichophyton rubrum. This study also ...

Evaluation of Antimicrobial Activity, Phytochemical Screening and Gas Chromatography-Mass/ Spectrophotometric Profile of Essential Oil from Persea americana & Citrus sinensis

Abstract This study aims to determine the phytochemical characteristics, structural determination of bioactive component from the leaf extract  using GCMs and experimentally investigation of the antibacterial activity of essential oil from Persea americana and Citrus sinensis leaf against seven bacteria (Gram-positive and Gram-negative) which are Staphylococcus aureus , Bacillus subtilis, Salmonella typhi, Klebsiella pneumonia, Escherichia coli, Proteus mirabilis and Pseudomonasaeruginosa kn...

Efficacy of Essential Oils from Persea Americana stem bark and Seed extracts

 The aim of the study is to evaluate the Antibacterial, Phytochemical analysis of Essential Oil, From the Stem Bark and seed extracts of Persea americana (Avocado). This was carried out by the crude extraction of the seeds and stem bark extracts with n-hexane. The extracts were used to determine the presence of Phytochemicals. Stock cultures of test organism such as Staphylococcus aereus, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, Proteus mirabilis were...

Assessment of Antibacterial, Phytochemical Properties and GCMS Profiling of Crude Polyalthia Longifolia Extract

Abstract The purpose of this present study is to determine  the  antibacterial  potency  of  essential  oil  of Polyalthialongifolia,  to  determine  the  presence  of phytochemicals and structural determination  of bioactive component  from  the  leaf  portion using  GCMS In  this present  study,  the  antibacterial  activity  from  the  essential oils of Polyalthialongifolia leaf and stem bark against seven bacteria  (gram-positive  and  gram-negative)  whic...

Assessment of Antimicrobial, Phytochemical Screening and Gas Chromatography-Mass Spectrophotometric Profile of Crude Chrysophyllum Albidum Essential Oil

Abstract The main purpose of this work is to investigate and compare the antimicrobial properties of Chrysophyllum albidum seed and stem bark essential oils, against some selected pathogenic isolates, to compare the phytochemical composition of the oil of Chrysophyllum albidum seeds and stem bark, to analyze the chemical compounds responsible for activities of the essential oil using Gas Chromatography – Mass Spectrometer (GC-MS) method in order to provide scientific validation for their us...

Assessment of Nephrotoxicity, Anti-Inflammatory and Antioxidant Properties

The purpose of this work is to assess the effect of nephrotoxicity, anti-inflammatory and antioxidant properties of epigallocatechin, epicatechin and stigmasterol phytosterol (synergy) isolated from ethyl acetate stem bark extract of Spondias mombin on wistar rats using molecular method of analysis. The selected biomarkers used for this work are GapdH, KIM-1, IL-1B, GSR, CAT, GPX-1 and IL-1Alpha at 30, 100 and 1000 mg/ml concentration. Seven week-old, male wistar rats from the Centre for Bioc...

Evaluation of Quantitative Elemental Compositions and Antioxidant Potentials of Spondias Mombin Extracts (Linn), A Precursor Against Infectious Diseases

The purpose of this research work is to investigate the elemental composition and antioxidant potentials of Spondias mombin (leaf, root and stem bark) at concentration of 10, 20 and 5mg/ml. using different conventional laboratory methods, also to determine the antioxidant potentials of Spondias mombin. The element present are Sodium (Na), Calcium (Ca), Magnesium (Mg), Zinc (Zn), Iron (Fe), Lead (Pb), Copper(Cu), Manganese (Mn), Potasium (K) and Phosphorus (P).it was observed that all elements...

Phenotypic identification and antibiogram profile of citrobacter species

The administration of antimicrobial agents on patients had a major impact on the rate of survival from infections. However, the evolving and the changing patterns of antimicrobial resistance posed a threat and caused a demand for review of the existing antibiotic policy or a demand for new antibacterial agents. This study was designed to determine the phenotypic identification in Citrobacter species and Antibiogram profile of citrobacter species isolated from University College Hospital (UCH)...

Bio-guided Isolation, Purification and Chemical Characterization of Epigallocatechin; Epicatechin, Stigmasterol, Phytosterol

Spondias mombin (Linn.) is a widely cultivated edible plant used in folkloric medicine for the treatment of severe infection and health disorders. This research work was carried out to isolation, purification and chemical characterization the bioactive constituents of the ethyl acetate stem bark fraction of Spondias mombin (Linn.), a medicinally important plant of the Anacardiaceae family. This study revealed the presence of flavonoid and steroids, which have been found to be important hormon...


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