Abstract This is a descriptive, retrospective case study conducted at research lab inSudan University of Science and Technology,department of histopathology during the period from January to September 2017The study aimed to assess the association between HPV-68 infection and cervical cancer among Sudanese patients through detection viral DNA using PCR technique, there by 40 formalin fixed embed tissue blocks of different cervical cancer type were collected. The tissue blockswere cut byRotary ...
Abstract The bacterium H.pylori is a fastidious, microaerophilic spiral Gram negative microorganism. This study aimed for molecular detection of metronidazole resistance genes (RdxA and FrxA) inH. pylori isolated from stool specimens in Elinjaz Diagnostic Center, in Khartoum State. A total of 65 samples were collected in this study, and bacteria were identified by the immune chromatography test (ICT), chemical method (Guanidine) was used for DNA extraction, and multiplex PCR was conducted to...
Abstract Diabetes is a group of metabolic disease characterized by hyperglycemia resulting from defect in insulin secretion, insulin action or both. Diabetic ketoacidosis ( DKA) is a type of diabetes complication lead to coma and death, the main characteristic of Diabetic ketoacidosis ( DKA) are: hyperglycemia and ketoacidosis. Urinary tract infection is the most type of infection in diabetic patients especially in females more than males. This study aimed to detect bacterial growth in diabet...
Abstract Streptococcus agalactiae (S.agalactiae) is one of the most important etiologic agent of several infection,cause abortion, and a present a major public health problem. This study aimed to detect the frequence of S. agalactiae among pregnant women with previous miscarriage by using PCR.Fifty samples of vaginal and high vaginal swabfrom pregnant women , were collected from different hospitals in Khartoum,25 from each vagina and high vagina. Patients’s data was collected by structured...
Abstract Tuberculosis still a major problem in Sudan and the anti tuberculosis drugs continue to be problematic in a systemic manner, which affect the liver leads to hepatotoxicity and elevation of liver parameter . The aim of the study to determine the levels of albumin and GGT in patients with tuberculosis under treatment and compare the level of albumin and GGT according to sex and type of drugs. This study was conducted at Kassala state during April to July 2017. In this study eighty blo...
Abstract Background: Menopause is a natural event in the ageing process and signifies the end of reproductive years with cessation of cyclic ovarian function as manifested by cyclic menstruation. Lipid profile is altered in menopause because of various reasons. Objectives: The aim of this study to evaluate cholesterol, triglycerides and lipase enzyme among Sudanese normal premenopausal women and postmenopausal women. Materials and methods: This is a case control study carried out in Saba’a ...
ABSTRACT An alarming increase in bacterial strains resistant to existing antibacterial agents demands a renewed effort to seek agents effective against pathogenic bacteria resistant to antibacterials. The aim of this study was to study the antibacterial activity of different concentrations of petroleum ether and methanol extracts of medicinal plant Nigella sativa (seeds) using cup-plate agar diffusion method on slected clinical isolates of bacteria. The study was conducted in Khartoum state,...
Abstract This is a cross sectional and case control study during the period from February to June 2013 to evaluate the level of HbA1c in type2 diabetic patients compared to fasting and random blood glucose. HbA1c test was determined by using Nyco Card Reader II (automated analyzer) with Nyco Card kits and glucose test (fasting and random plasma glucose) were determined by spectrophotometric semi-automated analyzer. With bio system kits reagents. Statistical analysis of the data was performed ...
Abstract Hepatitis B Virus infection is a major public health problem and causes majority of primary liver cancer. This was descriptive and cross sectional study aimed to evaluate the immune response after hepatitis B Vaccination in Medical laboratory Students. Study was conducted among Medical laboratory students in Omdurman state (Omdurman Islamic University) from April (2015) to May (2016). The blood specimens were collected and sera were separated and stored at - 20ºC until used, sera w...
Abstract This is a case control study conducted at Khartoum state during the period from April to October 2015. The aim of this study is to determine the complete blood cell count of Sudanese pregnant women at third trimester of pregnancy. Eighty healthy pregnant women and 40 non pregnant women were informed about the study and agreed for participation. A questionnaire was designed to collect information about the study group such as age, number of pregnancies and history of abortion in preg...
Abstract HBV remains a major global pathogen that cause acute and chronic hepatitis , liver cirrhosis , and hepatocellular carcinoma, IL-17 is pro-inflammatory cytokines play an important role in regulation of viral infections . This case-control study was aimed to evaluate the Interleukin-17(IL-17) level in Sudanese patients infected with Hepatitis B virus(HBV) compared to healthy control group in Khartoum state during period from March to October 2019. Sixty subjects, selected randomly in ...
Abstract This is a hospital-based analytical case-control study aimed to evaluate serum iron and vitamin B12 among Helicobacter pylori infected patients in Kingdom of Saudi Arabia, the study conducted during the period from January to July 2019. Under aseptic conditions 5 mL of venous blood was collected from 100 participants who selected conviniously (50 were H. pylori infected patients as cases and 50 apparently healthy individuals as controls). H. pylori screening for antibodies was detec...
Abstract A retrospective cross sectional molecular analysis for detection of epstein Barr virus on 51 breast cancer biopsies (paraffin embedded), previously diagnosed as breast cancer by histological examination, the samples were collected from the Military Hospital. This study was performed during the period from March to June 2017. According to their age, degree of differentiation, and type of cancer the women were divided into groups. The specimens were treated by xylene and the paraffin ...
Abstract This is an analytical retrospective case control hospital based study was conducted in Radiation and Isotope Center Khartoum (RICK), during the period from August 2016 to February 2017. The study aimed to detect Survivin expression in non-Hodgkin᾿s lymphoma among Sudanese patients using immunohistochemistry. Forty paraffin embedded blocks from patient’s samples previously diagnosed as non-Hodgkin᾿s lymphoma were collected. Samples include 26 (65%) malignant tumors, (including ...
Abstract Human parvovirus B19 infection can cause serious complications, especiallyamong pregnant women, patient with hematological disorders, immunocompromised and fetus,result in non-immune hydrops, intrauterine fetal death, and fetal death. This study aimedto detect thefrequency of B19V IgG andIgM antibodiesamong apparently healthy pregnant women attending selected antenatal care unitsin Khartoum state, Sudan. Blood specimens were collected during the period from September2018 toAugust 201...