Medical Lab Science and Tech Research Papers/Topics

Molecular Detection Of Mycobacterium Bovis In Cattle Milk And Abattoir Staff In Enugu

ABSTRACT The study was carried out to detect Mycobacterium bovis in cattle milk and abattoir staff in Enugu. Blood samples were collected from 50 subjects at Artisan and Ogbete market abattoirs with 25 samples from each location. Fifty Milk samples were collected from cattle at Fulani settlements which comprised of 13 from Gariki, 16 from 9th Mile and 21 from Monarch in Enugu. Milk samples were cultured on Lowenstein Jensen medium and incubated for 8weeks at 37o C. DNA was extracted from bot...

Epidemiology Of Klebsiella Pneumoniae Infection In Four Specialist Health Institutions In Enugu Urban, Enugu State, Nigeria

ABSTRACT In a comprehensive study conducted to determine the epidemiology of Klebsiella pneumoniae infection in four specialist health institutions in Enugu urban of Enugu State, Nigeria, a total of 564 cases were enlisted which involved 298 (52.8%) patients on admission in the hospitals, and 266 (47.2%) outpatients, aged 1 to 80 years. The study was carried out from October 2012 to March 2014. Sample collections for the study were based on the doctors’ assumption of diagnosis (from patien...

Physico-Chemical And Bacteriological Properties Of Water Supply Sited Around 9th Mile Brewery, Enugu State

ABSTRACT Analysis of the physico-chemical and bacteriological properties of Ajalli stream, Ngwo Spring water, Umuagba Ime-Owa River, Abonuzu stream and Ibute Amaeke hand dug well water all around 9th mile brewery in Enugu State were investigated between June 2009 and May 2010 to assess the extent of pollution of the water due to effluent discharges from the brewery industries around the studied area. These sites were investigated during the dry season and rainy season of the research period....

Serum Fibronectin Levels In People Living With Hiv/Aids

ABSTRACT Serum fibronectin level was investigated in people living with HIV/AIDS. A total of 161 subjects were recruited into the study. Out of this, 98 (60.9%) constituted the patient population while 63 (39.1%) formed the controls. The age range considered was between 10 to 65 years. Ethical clearance was obtained from the Health Research Ethics Committee (HREC) of University of Nigeria Teaching Hospital, (UNTH) Ituku Ozalla. Informed consent was obtained from the participants and question...

Molecular Characterization Of Exon 28 Of The Vwf Gene In Nigerian Population

Abstract von Willebrand’s disease is an autosomally inherited bleeding disorder caused by a qualitative and/ or quantitative abnormality of von Willebrand’s factor . It is one of the most common inherited bleeding disorders based on statistics from developed countries. Human von Willebrand’s factor is a large multimeric protein that plays an important role in the adhesion of platelets to the sub endothelium. It also acts as a carrier for coagulation factor VIII. Its deficiency and or d...

Serum Trace Element Status Of Psychiatric Patients At Federal Neuropsychiatric Hospital Enugu Nigeria

ABSTRACT A number of essential trace elements play major roles in various metabolic pathways. Deficiencies or alterations in the level of these trace elements adversely affect the ability to withstand oxidative stress-mediated cell damage. This study determined the serum status of copper, zinc and manganese in psychiatric patients. One hundred (100) psychiatric patients attending the Neuropsychiatric Hospital Enugu, Nigeria were categorized into two groups: fifty (50) patients on antipsychot...

Haemopoietic And Haematinic Potentials Of Crude Methanolic And Aqueous Seed Extract Of Telfairia Occidentalis In Albino Wistar Rats.

ABSTRACT Haemopoietic and Heamatinic potentials of crude methanol and aqueous seed extract of Telfairia Occidentalis (Ugu) in albino rats were studied using one hundred and twenty eight rats of mixed sexes ages 2-3 month weighing (150+ 20)g. Acute toxicity studies was performed and LD50 of the Telfairia Occidentalis obtained using standard methods. This work was carried out in the animal house of the University of Nigeria, Enugu Campus. The work was done in two set. The first set, the aque...

Serodetection of Syphilis among Recurrent Miscarriage and Non Miscarriage women in Wad Madani Teaching Hospital

This study was conducted among recurrent miscarriage and non miscarriage women in Wad madani teaching hospital to determine the prevalence of syphilis among those with previous miscarriage and those who had no miscarriage before . A total of ninety samples (n=90) were collected, during the period from April to June 2018, to demonstrate the frequency of syphilis and to compare between different diagnostic methods used for detection of syphilis. All samples were tested by using immunochro...

Frequency of Human Papilloma Virus (16 and 18) and Epstein Bar Virus among Sudanese patients with Esophageal Cancer using Immunohistochemistry and Polymerase Chain Reaction

This study was conducted as retrospective case study aimed to determine the frequency of HPV (16 and 18) and EBV among Sudanese with esophageal cancer using immunohistochemistry and PCR. The study included one hundred and two 102 paraffin blocks from patients previously diagnosed as esophageal cancer from Khartoum Sudan, in Ibn Sina hospital, khartoum hospital, soba teaching hospital, military hospital and national health laboratory. Their age ranged from 21 to 98 years old with mean ag...

Detection of- β lactamases Wide Spectrum Among Bacteria Isolated from Patients in Military Hospital, Omdurman

The emergences of extended-spectrum beta-lactamases (ESBLs) producing bacteria have important clinical and therapeutic implications. The present study was undertaken to detect extended spectrum beta lactamases among bacteria isolated from different clinical specimens. This study was carried out during period from April to August 2018. A total of 100 specimens were collected from patients admitted to Omdurman Military Hospital. These were from urine 73(73%), wound swab 20(20%) and sputum...

Investigations Of Haematopoietic Activities Of Sesamum Indicum, Linn Lectin In Albino Wistar Rats

ABSTRACT This study was designed, to investigate the haemopoietic activity of Sesamum indicum, Linn lectin (Beeniseed). The lectin was obtained and authenticated in the Botany department, University of Nigeria Nsukka. The LD50 of the lectin was determined with mice (n=17). Wistar rats (n=35), weighing 140 to 200 grams, aged 6 to 8 weeks were purchased and housed in the Animal House of College of Medicine, University of Nigeria Enugu Campus for 7 days to acclimatize before the study. The Wist...

Prevalence Of Hepatitis C Virus Infection In Diabetic Subjects At Enugu State University Of Science And Technology Teaching Hospital, Parklane, Enugu.

ABSTRACT The objective of this study was to assess the prevalence of anti-HCV antibodies amongst diabetics in Enugu. This is important as it has been indicated that presence of HCV in diabetics could lead to insulin resistance and thus increases diabetic complications. A total of 138 diabetics were recruited for this study, with a population control of 102 apparently healthy subjects, within the same age. A structured questionnaire was used to obtain information on the demographic, comorbid ...

Study the Impact of Physical Efforts on the Shoulder Tendons for the People with Different Jobs Using Ultrasounography

This was cross section study carried out in ultrasound department of Sudan university of science and technology (SUST) and kosti hospital , during the period from September 2018 to November 2018.MSKUS is consider as cost effective diadnostic modality to screen rotator cuff tears. Increase the thickness of rotator cuff tendons may indicate pathology ,there for the cut of point normal thickness should be known the question to be answer :can ultrasound be able to identify the normal thickn...

The Occurrence Of Hepatitis C Virus Infection In Apparently Healthy Subjects In Enugu Metropolis, Nigeria

ABSTRACT It is widely known that hepatitis C virus (HCV) slowly and progressively damages the liver while the human host may be unaware of the infection. The aim of this study was to investigate the prevalence of HCV in apparently healthy individuals in Enugu metropolis. This is important because it will explain the prevailing liver disease experienced in the society. A total of 214 apparently healthy individuals were recruited from Enugu North, Enugu South and Enugu East for this study. The...

Studies On The Extra-Human Distribution Of Enterohaemorrhagic Escherichia Coli From Borno And Enugu States Of Nigeria

ABSTRACT Enterohaemorrhagic Escherichia coli (EHEC) a causative agent of haemolytic uremic Syndrome (HUS) and haemorrhagic colitis especially in children, believed to originate from cattle is increasing with the day in parts of Nigeria. In order to ascertain the probable sources to assist a control regimen, laboratory analysis was carried out using standard bacteriological techniques for the isolation and identification of Escherichia coli (E.coli) and EHEC.A study was done on the distributi...


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