Natural & Applied Sciences

Research Papers/Topics Natural & Applied Sciences

ISOLATION AND IDENTIFICATION OF BACTERIA ASSOCIATED WITH WOUND SEPSIS

ABSTRACT The isolation and identification of bacteria associated with  150ml sepsis studies were carried out to 250 patient at National Orthopedic Hospital Enugu. (250) Two hundred and fifty patients with 150ml sepstis was grouped in to in patients and out patients.  The  sample collected are wound pus and wound exudates which were collected from patients with diabetic ulcer, surgery wounds, sickle cell ulcers, gun shot wound, using sterile stent striates to  aspirate the wound exudates ...

ISOLATION AND CHARACTERIZATION OF MICROORGANISMS FROM STORED PAP (OGI)

ABSTRACT The microbial changes which took place during the steeping and storage of pan (ogi) was studied.  The steeped water had an initial PH of 6.8 which latter reduced to 4.9 at the end of steeping.  The bacterial  number for the steeped water ranged from 4.7x104  to 3.2 x 107 cfu/ml while the fungal number ranged from 1.5 x103 to 5.7 x 106 cfu/ml.  In the stored pap, pap I had higher count which range from 7.2x104 to 6.0x107 cfu/g while 6.0x107 to 1.6x1011. The fungal count for pap ...

ISOLATION AND CHARACTERISATION OF BACTERIA ASSOCIATED WITH HAWKED SUYA- MEAT (CASE STUDY OF ENUGU)

ABSTRACT This project work is carried at the isolation and characterization of pathogenic bacteria from hawked suya meat,  were bought in full from the sellers and was collected in raps of aluminum foil. It was then taken to the laboratory for analysis the samples were homogenized and serial dilution of the sample was made out using 5 ml sterile pipette and test tubes. The dilution were  then cultured on different  media, macconkey agar, nutrient agar and blood agar end later incubated at...

GASTROENTERITIS IN PRIMARY SCHOOL CHILDERN IN ENUGU METROPOLIS (6 – 12 YRS)

ABSTRACT A total of fifty faecal samples were collected form pupils in airport primary school Emene Enugu were used for the study. The duration of the investigation was between February August 2005. These samples were  cultured and their causative organisms determined using macconkey agar, deocycholate citrate agar and an alkaline peptone water which was preparect according to manufacturers instructio. The species of organisms isolated include; escherichia coil 8  (29 %) Klebsiella 5 (18%)...

FUNGAL INFUSTATION ON BAKERY PRODUCT DREAM

ABSTRACT This project work which is all about the fungal infestation of bakery product BREAD, explain the kind or type of fungal organisms that are responsible for the  spoilage on contamination of bread. The infestation of fungal organism on bread depends on the kind and number of the agent present and upon the environment about them. this statement makes it clear that micro organism infest foods having suitable environment condition for their microbial activity. This project work is cente...

EXAMINATION OF INCIDENCE OF MALARIA INFESTATION CAUSED BY DIFFERENT SPECIES OF PLASMODIUM IN ENUGU METROPOLIS (A CASE STUDY OF PARKLANE HOSPITAL ENUGU)

ABSTRACT The incidence of the infestation of plasmodium species (P.Falciparum, P. Vivax,  P.Ovale, and P. alaria) which causes human malaria in Enugu metropolis was conducted at Parklane Hospital using their patients.  Thick and thin, smear of blood were made and examined after proper staining.  The staining method which proved effective were the Giemsa and Liesman staining method.  It was observed that P.Falciparum was most prevalent of the fair species while P.Ovale has occuarence.  T...

Evaluation Of Current Techniques In Diagnoses Of Human Immune deficiency Virus (HIV)

ABSTRACT A study was carried to ascertain the potency of some techniques used in diagnosis of Human Immunodeficiency virus (HIV).  Two hundred and five samples (203) were collected from the patients from the university of Nigerian teaching hospital and was analysed using Elisa kit, which has the ability to detect antibodies and antigens in the patients serum, the Western blots were used as the confirmatory test, to the Elisa test, In case where there is false positive of Elisa test, it is s...

COMPARATIVE ANALYSIS OF MICROBIAL LOAD OF THE ENUGU MAIN WATER PRODUCTION AND WATER AVAILABLE TO IMT CAMPUS II HOSTEL

ABSTRACT Samples of water from the different hostels in campus II and other source of water production namely Ajali-owa water, from bore hole 9th mile and spring water from GRA water board were water collecterd and analysed using total plate count and multiple tube fermentaton techniques coliform.  The results shows no coliforms in production water from Ajali-owa and in GRA spring water reservaiour and no coliform in 9th mile bore hole water, 14 organism and 149 cfu/ml plate counts were obt...

COMPARATIVE ANALYSIS OF ANTIMICROBIAL STRENGTH OF THREE MOST COMMON ANTIBIOTICS USED IN ENUGU (CASE STUDY OF ANTIBIOTICS DRUGS BROUGHT IN OBIAGU.)

ABSTRACT In comparing the antimicrobial strength of three most common antibiotics, which includes: streptomycin chloranphenicol and gentarccin.  Samples of urine and high vaginal swab (H.V.S) were collected from park lane Enugu. The organisms isolated were pure culture of staphylococcus aureus and Escherichia coli  Sensitivity test was carried out with  the use of sensitivity disk containing various minimum inhibitory concentration of the different antibiotics.  The result obtained shave...

ANAEMIA IN PREGNANCY (A CASE STUDY OF PREGNANT WOMEN ATTENDING ANTENATAL CLINIC AT UNIVERISTY OF NIGERIA TEACHING HOSPITAL (UNTH, ENUGU)

ABSTRACT The study on anaemia in pregnancy is aimed at determining packed cell volume (PCV), Haemoglobin (Hb) level and Erythrocyte sedimentation rate (ESR) of pregnant women, as well as the selferity, significance of maternal, age, educational level, occupation and gestational age to the occurrence of anaemia in pregnancy managed, diagnosed and admitted at the university of Nigeria teaching Hospital (UNTH) Enugu.  The result showed that most of the patient had moderate to secure anaemia an...

AN EPIDEMOLOGY SURVEY FOR SCTRISTOSOMIASIS AMONG PUPILS IN AMAGUNZE COMMUNITY IN NKANU LOCAL GOVERNMENT AREA IN ENUGU

ABSTRACT The survey of urinary sehistomiass in Ishele in Amagunze, Nkanu East local Government was carried out using two methods of diagnosis i.e combination of questionnaire on clinical signs and symptoms, and laboratory microscopy using primary school children in the area as test samples. A total of fifty samples were randomly collected reveals the prevalence of urinary Sdristomisis is 62 %. The risk factor attributed was found to cotain the sried of the type Bulinus (physopsis) globusus a...

DESIGN AND IMPLEMENTATION OF COMPUTER BASED POLICE INVESTIGATION SYSTEM (A CASE STUDY OF FIIB HEADQUARTERS OGBOR-HILL ABA)

ABSTRACT The world over, it is indisputable that the introduction of computer technology in different facets of life, has virtually transformed and entranced information processing, which is very vital in any organization or parastatals of government.  Not only as a means of information processing, the technology has also proved to be the fastest means of information retrieval, which forms the nucleus of this study. This work tries to x- ray the rate role of the computer in crime investigati...

DESIGN AND IMPLEMENTATION OF A COMPUTERIZED PROCEDURE FOR PRIVATIZATION OF A CORPORATE ENTERPRISE (A CASE STUDY OF NITEL LTD ENUGU)

ABSTRACT The focus on Privatization of Corporate enterprises is relatively recent in Nigeria.  The prominence in economic programs of most developing countries since the 1980’s is due to the need to reduce Public sector deficits and financial losses arising from gross inefficiencies of the public Enterprises.  Privatization attracts substantial amount of foreign investment inflows and new technological growth bringing about economic growth and reformation. The effective implementatio...

BACHERIOLOGICAL EXAMINATION OF IDODO RIVER

ABSTRACT This work was aimed at assessing microbial coal of Idodo river in Enugu state Nigeria. The samples of river was obtained from the arc of low activity, and a place where human activities are very high.  The samples were collected very early in the morning and afternoon.  They were examined to determined the bacterial organisms that are pathogenic to man when they drink it from the samples were inoculated into media.  Nutrient agar and macconkey agar media and incubate at the temper...

PREVALENCE STUDY OF HEPATITIS B (AUSTRALIAN ANTIGEN) AMONG PATIENTS IN NATIONAL ORTHOPAEDIC HOSPITAL ENUGU

ABSTRACT The prevalence of viral hepatitis B among patients in National Orthopedic Hospital Enugu was studied. The samples comprised that of men , woman and children 200 in numbers, all patient of orthopedic Hospital Enugu. Laboratory investigation done were this HBs  tested which 110 patient out of the 200 patients tested positive,  and liver function tests found abnormal in almost all the patients that tested positive to HBsAG routine test. The commonest clinical presentations were fever ...


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