Biochemistry Research Papers/Topics

Hepatoprotective Potential of Alkaloid Extracts from Vitex doniana and Ficus thonningii Leaves in Alloxan-induced Diabetic Rats

Aim: This study sought to evaluate the hepatoprotective potential of alkaloid extracts from V. doniana and F. thonningii leaves on alloxan-induced diabetic rats. Materials and Methods: Fresh leaves of V. doniana and F. thonningii were obtained from a local market Nkwagu in Abakaliki local government area of Ebonyi State. They were dried and pulverized to fine granules using manual grinder. Crude alkaloid was extracted using standard method. The acute oral toxicity of both plants was determine...

Ameliorative Potential of Bambara Nuts (Vigna subterranea (L.) Verdc) Extract against Acute Ethanol-Induced Oxidative Stress in Wistar Rats

Abstract The aim of this study was to determine the ameliorative potential of Bambara nuts extract against acute ethanol-induced oxidative stress in Wistar rats. Bambara nuts of the Songkhla 1 variety locally sourced in Obinze area of Owerri, Imo State, Nigeria were powdered using a coffee grinder and an extract obtained from a Soxhlet apparatus with methanol as solvent. Twenty-four adult male Wistar rats were acclimatized for seven days and were randomly divided into four groups of six rats ...

Evaluation of Methanolic Extract of Bambara Nut on Renal Indices of Wistar Rats

Background: Bambara nut is a common household food in Nigeria especially among the Igbos ofthe South Eastern part of the country.Aim: This study is aimed at investigating the effect of Bambara nut on renal indices of Wistar rats.Methodology: The Songkhla 1 variety (red seed coat) of Bambara nuts were locally sourced in Obinze area of Owerri, Imo State, Nigeria. The seeds were peeled and ground to a fine powder using a coffee grinder and extracted using soxhlet apparatus and methanol as the so...

Effect of Bambara Nut on Hepatic Biomarkers of Wistar Rats

Aim: This study sought to investigate the effect of Bambara nut on hepatic biomarkers of Wistar rats.Methodology: The Songkhla 1 variety (red seed coat) of Bambara nuts were locally sourced in Obinze area of Owerri, Imo State, Nigeria. The seeds were peeled and ground to a fine powderusing a coffee grinder and extracted using soxhlet apparatus and methanol as the solvent. Twentyfour adult male Wistar rats were acclimatized for seven days during which they were fed ad libitum with standard fe...

Effect of Bambara Nut Consumption on Blood Glucose Level

Aim: This study sought to investigate the effect of Bambara nut consumption on blood glucose level and lipid profile of Wistar rats.Methodology: The Songkhla 1 variety (red seed coat) of Bambara nuts were locally sourced from   Obinze area of Owerri, Imo State, Nigeria. The seeds were peeled and ground to a fine powder using a coffee grinder and extracted using soxhlet apparatus and methanol as the solvent. Twenty four adult male Wistar rats were acclimatized for seven days during which the...

Qualitative Determination of Phytochemical Composition of Ethanolic Extract of Xylopia aethiopica Fruit

Aim: The aim of this study is to qualitatively determine the phytochemical compositions of ethanolic extract of Xylopia aethiopica fruit.Methodology: The fruits of Xylopia aethiopica were obtained from new market in Aba, Abia State and were identified and authenticated by Prof. (Mrs) Margaret Bassey of the Department of Botany and Ecological Studies, University of Uyo with the voucher number UU/PH/4e. The plant was deposited in the Herbarium of the Department of Pharmacognosy and Natural Medi...

Hepatotoxic effect of Xylopia aethiopica Fruit in Wistar Rats

Background: Xylopia aethiopica fruit has been reported to possess high medicinal value. Thus, people use it without any regard for its toxicity.Aim: This study is therefore aimed at investigating its toxicity on the liver of Wistar rats.Methodology: The fruits of Xylopia aethiopica were obtained from new market in Aba, Abia State, Nigeria and were authenticated. They were air-dried and extracted using Soxhlet apparatus and ethanol as solvent. The median lethal dose (LD50) of the extract was d...

In Vitro Antioxidant Activities Of Dichloromethane Extracts Of Strychnos Henningsii G. And Ficus Sycomorus L.

Most of biochemical reactions in the body generates Reactive Oxygen Species (ROS), which are involved in the pathogenesis of oxidative stress-related disorders like diabetes, nephrotoxicity, cancer, cardiovascular disorders, inflammation and neurological disorders when they attack biochemical molecules like proteins, lipids and nucleic acid. Antioxidants are used to protect the cells or tissues against potential attack by ROS. Natural based antioxidants such as catalase, glutathione, superoxi...

Prevalence Of Hypoglycemia In Newborn At Kenyatta National Hospital And The Response To Glucose Supplementation In Low Birth Weight

Hypoglycaemia in neonates is defined as blood glucose concentration below 1.1mmol/l for term neonates. A common abnormality in neonates, it is associated, with neurological damage and death when it occurs in the first few days of life.  This is more pronounced in rural set-ups without the knowledge, detection and management facilities. When promptly diagnosed and managed, its effects can be prevented or minimized.  Studies at Kenyatta National Hospital (KNH) show that 59.8% of all neonates ...

In Vivo Anti-Inflammatory, Analgesic And Antipyretic Effects Of Dichloromethane Stem Bark Extract Of Acacia Mellifera In Mice And Rat Models

ABSTRACT  Inflammation is a reaction to irritants that causes an injury to the body. Pain is a physical discomfort caused by injury or illness while pyrexia is an increase in body temperature above the normal range (36.5°C–37.5°C). Commonly used conventional drugs against inflammation, pain and pyrexia are effective but due to long term use, these drugs tend to be costly and are associated with adverse side effects. Traditional medicine has been used as an alternative and complementary d...

Antiproliferative Activities, Gene Expression Profiles And Toxicity Of Selected Medicinal Plants Against Cancerous And Normal Cells

Cancer is among the leading causes of morbidity and mortality worldwide and accounts for up to 28,000 deaths annually in Kenya. Globally, approximately 13% of all deaths annually are attributed to cancer. Surgery, radiation and chemotherapy are the current treatment techniques for cancer; however, these methods are expensive, have high failure rates and have been associated with detrimental side effects. Plants derived products could be good candidates in alleviating challenges being exp...

Correlation Between High Density Lipoprotein Cholesterol And Kidney Function In Type 1 Diabetes Mellitus As A Predictor Of Diabetic Nephropathy In Human Subjects

ABSTRACT Diabetic nephropathy (DN) is a common microvascular complication occurring five years from onset of type 1 diabetes mellitus, leading to renal failure and death. It is characterized by albuminuria and a reduced glomerular filtration rate. Although much emphasis has been placed on screening for albuminuria and estimated glomerular filtration rate (eGFR) to predict or diagnose DN, less attention has been focused on the role of high density lipoprotein cholesterol (HDL-C) in risk asses...

In Vivo Hypoglycemic Activity And Safety Of Selected Medicinal Plants Used In The Management Of Diabetes Mellitus In Elgeiyo-Marakwet County, Kenya

ABSTRACT  Diabetes Mellitus is a group of metabolic disorders sharing a common underlying feature of hyperglycemia with disturbances of carbohydrate, protein and fat metabolism. The disease may present with characteristic symptoms such as polydipsia, polyuria, polyphagia, blurring of vision, and weight loss and in its most severe forms, ketoacidosis or a non-ketotic hyperosmolar state may develop and lead to stupor, coma and, in absence of effective treatment, death. Hyperglycemia may be as ...

Biochemical Effects Of Sewage Pollution On The Benthic Organism Nerita Polita

ABSTRACT Rapid urbanization has resulted into substantial increase in quantities of sewage, which is traditionally discharged into the ocean, causing a number of environmental problems including threats to oceanic lives as well as ground water contamination. So far, there is limited data in Kenya regarding the toxic effects of sewage on benthic organisms. The aim of this study was to investigate the biogeochemical effects of sewage pollution on the mollusc Nerita polita by determining its im...

Comparative And Functional Analysis Of Tsetse Fly Aquaporins

ABSTRACT Tsetse flies (Glossina sp) are the vector of African trypanosomes, the causative agents of sleeping sickness in man and Nagana in cattle. The insect is strictly hematophagous, taking in significantly high quantities of water during bloodmeals that present a considerable osmotic challenge. This necessitates machinery for eliminating excess water. One potential candidate is a group of membrane channel proteins called aquaporins (AQPs) that are responsible for the movement of water acr...


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