Microbiology Research Papers/Topics

ALLELIC AND GENOTYPIC FREQUENCIES OF THE ABO BLOOD GROUP SYSTEMS AND THEIR ASSOCIATIONS WITH Helicobacter pylori INFECTION AMONG PATIENTS WITH GASTROINTESTINAL COMPLAINTS IN EJERE DISTRICT,WE

Abstract: Helicobacter pylori is a small, spiral-shaped bacteria that lives on the surface of the stomach and duodenum. H. pylori is closely associated with onset of diseases such as gastric ulcers, duodenal ulcers, gastritis and gastric cancers. The aim of this study was to determine the allelic and genotypic frequencies of ABO blood group system and their associations with H. pylori infection among 384 patients with gastrointestinal complaints in the Ejere and Amaro Helth Center. Stool sam...

PHYTOCHEMICAL ANALYSIS AND EVALUATION FOR ANTIMICROBIAL ACTIVITIES OF THE EXTRACTS OF Psidium guajava L. AND Calpurnia aurea (Ait.) Benth, AGAINST SELECTED HUMAN BACTERIAL PATHOGENS

Abstract: Plants are rich in a wide diversity of secondary metabolites which have been found to exhibit antimicrobial, antioxidant and antitumor activities. Guava (Psidium guajava L., Myrtaceae) and (Calpurnia aurea L. Fabaceae) are known for their traditional medicinal values. These studies were conducted with the objective of analyzing the secondary compounds profile of the two plant species and evaluate their antimicrobial properties on selected human bacterial pathogenes. Extraction was ...

ANTIMICROBIAL AND ANTIOXIDANT PROPERTIES OF ENDOPHYTIC FUNGI FROM PINEAPPLE FRUIT, IN GEDEO ZONE, SOUTHERN ETHIOP

Abstract: The pineapple fruit is a component of the traditional medicine, it is also known with excellent quality, special flavor, and nutritional richness, is favored by consumers worldwide. The pineapple fruit juice contributes to healthy living, because it is good source of vitamins, phenols, organic acids and carbohydrate. The aim of this research was to conduct study on antimicrobial and antioxidant activities of endophytic fungi from pineapple fruit using isolation method. The experime...

MICROBIAL CONTAMINATIONS OF MEAT AND ASSOCIATED RISK FACTORS IN BEKOJI TOWN, ARSI ZONE, OROMIA REGIONAL STATE, ETHIOPIA

Abstract: The consumption of raw meat has associated with cultural practices and widespread raw beef consumption habit that can be a potential source for food borne illnesses in Ethiopia. Therefore, the present study was intended to assess microbiological contamination and associated risk factors in Bekoji town. A cross sectional study was employed whereby a simple random sampling of Butcher shops in Bekoji Town. The cattle (muscle, liver, and intestine) meat samples were collected aseptical...

ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ENDOPHYTIC FUNGI FROM FENNEL (Foeniculum vulgare Mill.) LEAF AND SEED

Abstract: Antioxidant and antimicrobial properties are the biological activity of natural compoundsisolated from medicinal plants that are rich source of novel drugs. Endophytic fungi are a potential source of novel bioactive compounds. The present study investigates antimicrobial and antioxidant properties of endophytic fungi from fennel (Insilali) seeds and leaves. The antioxidant activity was conducted based on determination of phenolic content, 1, 1-diphenyl-2-picrylhydrazyl and hydrogen...

ANALYSIS OF CHEMICAL COMPOSITION OF CACTUS PEAR (OPUNTIA FICUS-INDICA L.) CLADODE EXTRACT AND ITS ROLES IN THE PREPARATION OF FUNGAL CULTURE MEDIA

Abstract: The present study was aimed at analyzing the chemical composition of the crude extract of cactus pear (Opuntia ficus-indica L.) cladode and examine its suitability for fungal culture media. For this purpose cactus pear cladode samples were collected from Haramaya University campus Model School area. The chemical composition analysis was done according to standard procedures. The growth of five fungal species (R. stoloinfer ATCC 1052, P. digitatum ATCC 2560, A. niger ATCC 1232, T. a...

ISOLATION OF MICROORGANISMS (FUNGI) FROM KOCHO AND BULLA FOR ANTIOXIDANT ACTIVITIES

Abstract: Enset (Ensete ventricosum (Welw.) Cheesman) belongs to the family Musaceae, and is one of the oldest cultivated herbaceous and monocarpic banana-like food security crops in Ethiopia. Microbes which colonize living internal tissues of plants without causing any harm to their host can be used for testing their antioxidant potential under controlled conditions. These microbes protect their hosts from adverse conditions by secreting bioactive secondary metabolites. Therefor, the presen...

PHYSIOCHEMICAL AND MICROBIAL QUALITY OF DRINKING WATER, A CASE OF NEGELE ARSI WEREDA, WEST ARSI ZONE, OROMIA REGIONAL STATE, ETHIOPIA

Abstract: Water is natural resources and is essentially to sustain life. Poor drinking water quality has been linked to several diseases of man particularly in developing countries, where availability of potable water is a big challenge. Thus, this study was conducted to assess the biological and physico-chemical quality of drinking water at NegaleArsiWoreda.A total of 72 water samples were collected from deep well water. The analysis demonstrated that all the water samples in the study area...

SCREENING AND CHARACTERIZATION KERATINASE ENZYME FROM BACTERIA IN KERATINOUS WAST

Abstract: Microbial keratinases are keratinolytic enzymes widely used in many industrial processes and management of wastes. This study was conducted with the aim of isolation of efficient keratinase producing bacteria form the soils of chicken feather-dumping sites and human hair-dumping places, determining optimum keratinase production conditions and partially characterizing the stability and activity of keratinase with regards to some physicochemical parameters. Plating of soil sample sus...

PRODUCTION OF CELLULASE USING FUNGAL ISOLATES FROM KOCHO (Ensete ventricosum (Welw.) Cheesman)

Abstract: Cellulases are the type of enzymes which catalyze the hydrolysis of cellulose and related oligosaccharide derivatives, is considered a potential tool for industrial saccharification of cellulosic biomass. The production of bio-based products and bioenergy from less costly renewable lignocellulosic materials would bring benefits to the local economy, environment, and national energy security. The processed enset (kocho and bulla) are rich in carbohydrates, and fiber and some mineral...

ANTIOXIDANT AND ANTIMICROBIAL POTENTIALS OF ENDOPHYTIC FUNGIFROM(Lippia adoensis Hoechst.Var. Koseret)LEAF AND SEED

Abstract: Endophytic fungi represent a wide diversity of microbial adaptations that have evolved in special and unusual environments, making them a great source of study and research for new drugs for medical, industrial, and agriculture uses.The present study was undertaken to investigate antioxidant and antimicrobial potential of endophytic fungalextracts ofkoseret leaf and seeds.The leaf and seed samples collected from the home gardens were used for endophyte fungal extraction.Endophytes ...

ISOLATION AND CHARACTERIZATION OF THE DOMINANT YEAST FROM INGREDIENTSOF TELLA, THE TRADITIONAL FERMENTED ALCOHOLIC BEVERAGE

Abstract: Tella” is one of the important Ethiopian traditional beverages, which is prepared from different ingredients. Ingredients of tella are one of the major important sources of the isolation of dominant yeast from the local alcoholic beverages. Hence, this study was conducted to determine the major source of dominant yeast cells by identifying them in the ingredients of tellasuch as; gesho, bikil, tense, difdif and compared within that of bakery yeast. The collected samples were proc...

PRODUCTION OF BIOACTIVE COMPOUNDS WITH ANTIOXIDANT AND ANTIMICROBIAL POTENTIALS BY ENDOPHYTIC FUNGI FROM RED GRAPE (Vitis vinifera L.) FRUIT PEEL AND SEED

Abstract: Grapes (Vitis vinifera L.) are among the most widely consumed fruits for its asociated health benefits. Endophytic fungi are groups of fungi with very specific ecosystem inside plant tissues and produces varieties of secondary metabolites with medicinal and nutraceutical applications. The present study was undertaken to investigate production of bioactive extracts with antioxidant and antimicrobial potentials activities of endopytic fungi from red grape peel and seed. The antioxida...

ISOLATION AND IDENTIFICATION OF POTENTIAL INDIGENOUS BACTERIA USEFUL FOR BIOREMEDIATION OF BREWERY EFFLUENTS

Abstract: The brewery industry consumes daily a large volume of water and chemicals and generates a high amount of nutrient-rich wastewater. The discharge of this wastewater into the environment without proper treatment causes serious and prolonged ill consequences on the ecosystem. Thus, the objectives of this study were to assess the physicochemical characteristics of Harar Brewery effluent; to isolate, identify and determine the efficiency of the indigenous bacteria in removing or reducin...

ISOLATION AND ANTIMICROBIAL SUSCEPTIBILITY TEST OF NON TYPHOIDAL SALMONELLA FROM RAW COW MILK AND ASSESSMENT OF HYGIENIC PRACTICES IN GURSUM DISTRICT, EASTERN HARARGE, ETHIOPIA

Abstract: Non-Typhoidal Salmonella represents an important human and animal pathogen world-wide. Most human non-typhoidal Salmonella (NTS) cases are foodborne, but each year infections are also acquired through direct or indirect animal contact. Epidemiological information on the prevalence of Salmonella is a prerequisite to develop appropriate control strategies. A cross sectional study was conducted from July 2022 to January 2023 to isolate and perform Antimicrobial susceptibility test of ...


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