Abstract Coronavirus is a disease caused by a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which emerged as a global pandemic in 2019 from Wuhan, China. Since its emergence, it has caused immense suffering to human life, 6.27 million lives have been lost, movement curtailed and social dynamics disrupted. The golden standard for getting samples for SARS-CoV-2 detection is through oral- nasopharyngeal swab, this method of sample collection is invasive and uncomfortable, thus st...
Abstract/Overview Malaria continues to place a disease burden on millions of people throughout the tropics, especially in sub-Saharan Africa. Although efforts to control mosquito populations and reduce human-vector contact, such as long-lasting insecticidal nets and indoor residual spraying, have led to significant decreases in malaria incidence, further progress is now threatened by the widespread development of physiological and behavioural insecticide-resistance as well as changes in t...
Abstract/Overview Background: Everyday hundreds of people, mainly men, set out to take part in a vibrant artisanal capture fshing (ACF) industry on Lake Victoria. It is not known whether actions of artisanal fshers, in their unrelenting quest for exist ence, surpass ecosystems’ sustainability thresholds with potentially negative repercussions on human health with respect to malaria transmission potential. This article sought to fll this information gap. Methods: This study used an ecosy...
Abstract Paenibacillus larvae is a spore-forming bacterium causing American foulbrood (AFB) in honey bee larvae. The remains of a diseased larva contains billions of extremely resilient P. larvae spores viable for decades. Burning clinically symptomatic colonies is widely considered the only workable strategy to prevent further spread of the disease, and the management practices used for decontamination requires high concentrations of chemicals or special equipment. The aim of this study was...
Abstract/Overview We identified 672, 374, and 379 CYPs proteins encoded by the CYPs genes in Gossypium hirsutum, Gossypium raimondii, and Gossypium arboreum, respectively. The genes were found to be distributed in all 26 chromosomes of the tetraploid cotton, with chrA05, chrA12, and their homeolog chromosomes harboring the highest number of genes. The physiochemical properties of the proteins encoded by the CYP450 genes varied in terms of their protein lengths, molecular weight, isoelectr...
Abstract Two new norhopane derivatives namely 3β,6β,22-trihydroxy-7β,11α-di[(4-hydroxybenzoyl)oxy]-21αH-24- norhopa-4(23)-ene (1) and 3β,6β,22-trihydroxy-7β-[(4-hydroxybenzoyl)oxy]-21αH-24-norhopa-4(23)-ene (2) together with two previously reported compounds, including a norhopane, 3β,6β,11α-trihydroxy-7β-[(4-hydroxybenzoyl) oxy]-24-norhopa-4(23),17(21)-diene (3) and a norneohopane, (21αH)-24-norneohopa-4(23), 22(29)-diene-3β,6β,7β-triol 7-caffeate (4) were isolated from the...
Abstract Diversity patterns of tropical alpine vegetation is poorly studied. We estimated vascular plant floristic richness for two typical afro-alpine communities in Mount Kenya using a series of nested plots sized from 25 to 100 m2. The α-diversity was low for all plot sizes (4.8–7.8 and 12.8 species per one and 100 m2, respectively). Comparative analysis of α-, β- and γ-diversity across 22 plant communities from five Mountain regions (Mount Kenya, European Alps, Caucasus, Tibet, New...
Abstract Camels are increasingly becoming the livestock of choice for pastoralists reeling from effects of climate change in semi-arid and arid parts of Kenya. As the population of camels rises, better understanding of their role in the epidemiology of zoonotic diseases in Kenya is a public health priority. Rift Valley fever (RVF), brucellosis and Q fever are three of the top priority diseases in the country but the involvement of camels in the transmission dynamics of these diseases is poor...
Abstract/Overview Approximations of fixed points have been done in different space and classes. However, characterizations in norm attainable classes remain interesting. This paper discusses approximation of nonexpansive operators in Hilbert spaces in terms of fixed points. In particular, we prove that for an invariant subspace H0 of a complex Hilbert space H; there exists a unique nonexpansive retraction R of H0 onto _(Q) and x 2 H0 such that the sequence f_ng generated by _n =_nf(_n)+(1...
Abstract Lake Magadi is an alkaline saline Lake in Kenya, which lies on the Great Eastern Rift valley. Although the lake is characterized by extremes of salt, pH and temperature, it supports diverse groups of cyanobacteria. In this study, we used different media to isolate novel groups of cyanobacteria. We recovered 11 isolates affiliated to the orders Chroococcales, Oscillatoriales, Pleurocapsales and Nostocales. Isolates affiliated to Chroococcidiopsis species had similarity values below 9...
Abstract Mycobacterium abscessus is intrinsically resistant to most antimicrobial agents. The emerging infections caused by M. abscessus and the lack of effective treatment call for rapid attention. Here, we intended to construct a selectable marker-free autoluminescent M. abscessus strain (designated UAlMab) as a real-time reporter strain to facilitate the discovery of effective drugs and regimens for treating M. abscessus. The UAlMab strain was constructed using the dif/Xer recombinase sys...
Abstract Lipids are hydrocarbons comprised of long‐chain fatty acids and are found in all living things. In the environment, microorganisms degrade them to obtain energy using esterases and lipases. These enzymes are nowadays used in different industrial applications. We report isolation of 24 bacteria with esteresic and lipolytic activity from Lake Magadi, Kenya. The isolates were characterised using morphological, biochemical, and molecular methods. Isolates grew at an optimum salt conce...
Abstract Background: Total community rDNA was used to determine the diversity of bacteria and archaea from water, wet sediment and microbial mats samples of hot springs in the Eastern lowlands of Eritrea. The temperatures of the springs range from 49.5 °C to 100 °C while pH levels varied from 6.97 to 7.54. Akwar and Maiwooi have high carbonate levels. The springs near the seashore, Garbanabra and Gelti, are more saline with higher levels of sodium and chlorides. Elegedi, situated in the Al...
Abstract Background and Objective: Tropical maize is the most cultivated crop in sub-Saharan Africa and is a staple food to over 220 million people. This study, evaluated the competence of four tropical maize (Zea mays L.) inbred lines to callus induction and regeneration through somatic embryogenesis when co-cultivated on yeast extract peptone medium (YEP). Materials and Methods: Transient GUS assay was used to evaluate the competence of the genotypes to Agrobacterium-mediated transformatio...
Abstract Eritrea has a number of hot springs whose physicochemical characteristics are not documented. This study examined the thermal and chemical features of five hot springs located in the eastern escarpment of Eritrea. Field data and water samples were collected from five hot springs namely; Akwar and Maiwooi near Gahtelai, Garbanabra and Gelti near Irafayle at the Gulf of Zula and Elegedi in Alid volcanic center. The water temperatures at source varied from 49.5°C to 100°C while pH le...