Natural & Applied Sciences

Natural & Applied Sciences Research Papers/Topics

Mass developments of a small sized ecotype of Arthrospira fusiformis in Lake Oloidien, Kenya, a new feeding ground for Lesser Flamingos in East Africa

Abstract The Lesser Flamingos constantly commute between the soda lakes of East Africa in search of food. Their preferred food, the cyanobacterium Arthrospira fusiformis, usually establishes dense populations in saline–alkaline habitats. However, the algal food resources are not stable and crash unexpectedly from time to time. Hence any site that provides food in suitable quality and quantity makes a valuable contribution to the survival of these nearly endangered birds. This paper reports...

Desertifilum fontinale sp. nov. (Oscillatoriales, Cyanobacteria) from a warm spring in East Africa, based on conventional and molecular studies

Abstract Desertifilum is a filamentous cyanobacterium of crusts and biofilms, recently described from the extreme hot and dry Thar Desert in north–western India. A new species was isolated from a warm spring near Lake Bogoria, Kenya and was characterised by light and electron microscopy and phylogenetically using the 16S rRNA gene, beta and alpha subunits including intergenic spacer (cpcBA–IGS) and 16S–23S internal transcribed spacer (ITS). The aquatic habitat of the new material from ...

An overview of advances in bioinformatics and its application in functional genomics

Abstract Bioinformatics is the scientific discipline that is concerned with the efficient management and useful interpretation of large scale biological information. Functional genomics aims at mapping DNA sequences and the components they encode for, to the function they perform. Initial efforts in bioinformatics were focused on the analysis of DNA sequence data. Presently, the scope and objectives of bioinformatics research and development have been broadened owing to the accelerating gene...

454 Pyrosequencing‑based assessment of bacterial diversity and community structure in termite guts, mounds and surrounding soils

Abstract Termites constitute part of diverse and economically important termite fauna in Africa, but information on gut microbiota and their associated soil microbiome is still inadequate. In this study, we assessed and compared the bacterial diversity and community structure between termites’ gut, their mounds and surrounding soil using the 454 pyrosequencing-based analysis of 16S rRNA gene sequences. A wood-feeder termite (Microcerotermes sp.), three fungus-cultivating termites (Macroter...

Haloleptolyngbya alcalis gen. et sp. nov., a new filamentous cyanobacterium from the soda lake Nakuru, Kenya

Abstract The food web of the saline-alkaline Lake Nakuru is dominated by the cyanobacterium Arthrospira fusiformis as the primary producer and a huge population of Lesser Flamingos as direct consumers. However, the dense blooms of Arthrospira are not stable, and collapse irregularly and unpredictably. During such periods they are replaced by other algae or cyanobacteria. The wide fluctuation in the cyanobacterial and algal populations of Lake Nakuru has a great influence on food availability...

Potential of indigenous bradyrhizobia versus commercial inoculants to improve cowpea (Vigna unguiculata L. walp.) and green gram (Vigna radiata L. wilczek.) yields in Kenya

Abstract Limited information is available on reduced cowpea (Vigna unguiculata L. Walp.) and green gram (Vigna radiata L.Wilczek.) yields in Kenya. Declining soil fertility and absence or presence of ineffective indigenous rhizobia in soils are assumptions that have been formulated but still require to be demonstrated. In this study, soils were collected from legume growing areas of Western (Bungoma), Nyanza (Bondo), Eastern (Isiolo), Central (Meru) and Coast (Kilifi) provinces in Kenya to a...

Hidden diversity of eukaryotic plankton in the soda lake Nakuru, Kenya, during a phase of low salinity revealed by a SSU rRNA gene clone library

Abstract A SSU rRNA gene clone library was constructed to establish the diversity of eukaryotic plankton in the African soda lake Nakuru during a phase of low salinity (9.7 ppt = hyposaline). Normally, the lake is mesosaline (up to 50 ppt) and its phytoplankton is dominated by few species of cyanobacteria, in particular Arthrospira fusiformis, which is the main food resource of Lesser Flamingos. Our study recovered a unique phytoplankton species composition characterized by a high diversity ...

Isolation and characterization of bacillus species from soil in Ngere tea catchment area of Murang’a county, Kenya

Abstract Bacteria are a very diverse group of organisms in soil, and major taxonomic groups are represented in most soils. The extent of the diversity of microorganisms in soil is seen to be critical to the maintenance of soil health and quality, since a wide range of bacteria are involved in the important soil functions. The objectives of this study were to isolate, characterise and identify groups of bacteria from Bacillus species that are associated with soil quality in tea growing areas ...

Isolation and characterization of bacteria isolates from soil feeding termites and soil from Juja and Kakamega forest in Kenya

Abstract In the last several years information on the gut ecosystem of termites has continued to be gathered. Most studies have been focused on wood feeding termites but studies on soil feeders remain sparse owing to their difficulty of establishing permanent laboratory cultures. The aim of this study was to isolate, characterize and identify bacteria resident in the soil feeding termite gut, mound and parent soil of Cubitermes species with the potential to produce antibiotics and enzymes fo...

Cultural Characterization of Fungi Isolated From Oil Contaminated Soils

Abstract Application of Fungi for effective removal of hydrocarbon contamination from soil is being considered as the better option when it comes to biodegradation. Other method like physical and chemical bioremediation leads to production of toxic compounds and these methods are not cost effective. In the present study, soil samples from four different oil contaminated soils were assessed for any recovery of fungi present. Cultural characterization was used as preliminary identification usi...

Local management and landscape drivers of pollination and biological control services in a Kenyan agro-ecosystem

Abstract Arthropods that have a direct impact on crop production (i.e. pests, natural enemies and pollinators) can be influenced by both local farm management and the context within which the fields occur in the wider landscape. However, the contributions and spatial scales at which these drivers operate and interact are not fully understood, particularly in the developing world. The impact of both local management and landscape context on insect pollinators and natural enemy communities and...

Genetic Transformation of Sweet Potato for Improved Tolerance to Stress: A Review

Abstract The sweet potato (Ipomoea batatas Lam) is a major staple food in many parts of the world. Sweet potato leaves and tubers are consumed as food and livestock feed. Biotic and abiotic stresses affect yield leading to a reduction in production. This review analyzes factors limiting sweet potato production and the progress made towards stress tolerance using genetic transformation. Genetic transformation could enhance yield, nutritional value and tolerance to stress. Transgenic sweet pot...

Seagrass Removal Leads to Rapid Changes in Fauna and Loss of Carbon

Abstract Seagrass habitats are important natural carbon sinks, with an average of ∼14 kg C m−2 buried in their sediments. The fate of this carbon following seagrass removal or damage has major environmental implications but is poorly understood. Using a removal experiment lasting 18 months at Gazi Bay, Kenya, we investigated the impacts of seagrass loss on sediment topography, hydrodynamics, faunal community structure and carbon dynamics. Sediment pins were used to monitor surface elevat...

Drought Response in Selected Tropical Inbred Maize Lines and Relative Expression of PARP2 Gene under Limited Water Conditions

Abstract Drought is the leading single factor that limits maize production thus inhibiting the crops genetic potential. In response to drought, maize and other plants synthesize Poly ADP-Ribose (PAR) protein. This process is controlled by the Poly ADP-Ribose Polymerase (PARP) genes and consumes cellular energy, leading to plant death. This study evaluated four tropical inbred maize (Zea mays L.) lines; CML 216, CML 144, A04 and E04 for their response to growth limiting water stress and their...

Carbon in the Coastal Seascape: How Interactions Between Mangrove Forests, Seagrass Meadows and Tidal Marshes Influence Carbon Storage

Abstract Purpose of Review We use the ‘seascape’ concept to explore how interactions between mangrove forests, tidal marshes and seagrass influence the storage of carbon in these ecosystems. Mangrove forests, with the other two ‘blue carbon’ habitats, are exceptionally powerful carbon sinks. Maintaining and enhancing these sinks is an emerging priority in climate change mitigation. However, managing any one ecosystem on its own risks is ignoring important contextual drivers of carbon...


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