Biochemistry Research Papers/Topics

EFFECTS OF DIFFERENT LEVELS OF BLACK CUMIN SEEDS (Nigella sativa L.) AS AN ADDITIVE ON LAYERS PERFORMANCE AND BLOOD BIOCHEMICAL CONSTITUENTS

Abstract: The study was conducted to investigate the effects of different levels of black cumin seed (Nigella sativa L.) on chicken performances, fertility and hatchability of egg, chick quality, embryonic mortality, blood parameters and economic benefit of black cumin seeds on white leghorn layers at Haramaya University Poultry Farm for 70 days. One hundred eighty WL layer at twenty- four weeks of age with an average initial body weight of 1219.52± 5.45g were randomly distributed to four t...

Effectiveness of using milled Moringa Oleifera seeds as a disinfectant in waste water treatment

Abstract: Water disinfection is a crucial water treatment process because it deals with the removal of pathogens to make water safe to drink. Moringa oleifera extract was used as a disinfectant and the total coliform count was monitored through microscopic observations. The total coliform bacteria removal at a dosage of 5 ml/100 ml of Moringa Oleifera extract was counted to be 13 colonies from an original count of 200 colonies, translating to about 93.5% removal of the bacterial strains. The...

Optimizing yield and quality of bio-oil: a comparative study of Acacia tortilis and Pine dust

Abstract: We collected pine dust and Acacia tortilis samples from Zimbabwe and Botswana, respectively. We then pyrolyzed them in a bench-scale plant under varying conditions. This investigation aimed to determine an optimum temperature that will give result to maximum yield and quality of the bio-oil fraction. Our experimental results show that we obtain the maximum yield of the oil fraction at a pyrolysis temperature of 550 ◦C for the acacia and at 500 ◦C for the pine dust. Our results ...

The sustainability of biodiesel synthesis from different feedstocks: a review

Abstract: Currently, the world is facing an energy crisis due to an increase in the global energy demand and this has led to an increased dependence on fossil fuels for energy generation and transportation. The steady rise in the world population which is more than seven billion currently is met with an increase in fossil fuel consumption thereby increasing the threat of fossil depletion and an apparent energy deficit. Fossil fuel emissions pose a threat to the environment and the general hu...

Synthesis, characterization of nitro or amino substituted pyridyl ligands bridged by an ester or ether bond, and their antibacterial assessment against drug resistant bacteria.

Abstract: A series of ester and ether bridged compounds bearing the nitro and amino moieties were synthesized and characterized, in order to assess their ability to potentially serve as antibacterial agents against drug resistant bacteria. All the compounds were obtained at considerable yields while characterization techniques confirmed their successful synthesis. Furthermore, their antimicrobial assessments showed that all the compounds exhibited antibacterial activity against the Gram-nega...

A computational study of the s2 state in the oxygen-evolving complex of photosystem II by electron paramagnetic resonance spectroscopy

Abstract: The S2 state produces two basic electron paramagnetic resonance signal types due to the manganese cluster in oxygen-evolving complex, which are influenced by the solvents, and cryoprotectant added to the photosystem II samples. It is presumed that a single manganese center oxidation occurs on S1 → S2 state transition. The S2 state has readily visible multiline and g4.1 electron paramagnetic resonance signals and hence it has been the most studied of all the Kok cycle intermediate...

Polyphenols as Potential Indicators for Drought Tolerance in Tea (Camellia sinensis L.)

Abstract: Plant polyphenols have gained prominence in quality of plant products and in human health. an experiment...(continue from the attached pdf)

Characterization and Functional analysis of tricorn interacting factor 3 in Trypanosoma brucei brucei as potential drug targets against African Trypanosomiasis

Abstract: Trypanosomes are protozoans causing African trypanosomiasis, a neglected tropical disease in Africa affecting humans and animals. Despite advancement in African Trypanosomiasis research, the disease continues to threaten millions of people and animals in Sub-Saharan Africa. Control methods have focused on the use of drugs which have adverse effects and vector control methods which have proved to be ecologically unsustainable while vaccines are still not available due to antigenic v...

Vitellogenin of the Tobacco Hornworm, Manduca Sexta: Properties and Endocytotic Incorporation into follicles

Abstract: Manduca sexta vitellogenin is a phosphoglycolipoprotein (Mr - 500,000) that contains two copies of the apoproteins (apovitellogenin-1, Mr 180,000 and apovitellogenin-11, Mr 45,000), 13 percent lipids, 3 percent carbohydrates and 0.6 percent phosphorus. The two apoproteins are immunologically distinct polypeptides and apovitellogenin-11 is not completely accessible to the aqueous environment in the intact molecule. The carbohydrate moiety located on apovitellogenin-1 has a high mann...

Isolation of Glossina Typanolysis/Trypanoglutinin and Assessment of their use in the Interruption of Trypanosome Differentiation

Abstract: Midgut trypanolysin from Glossina morsitans morsitans was isolated by a combination of an anion-exchange and gel permeation chromatography. The trypanolysin activity was recovered in the bound fraction. The native molecular weight of trypanolysin was determined -669kDa. Analysis oftrypanolysin by SDS-PAGE recovered a single sub-unit ofMr-14kDa. The induction of trypanolysin activity by bloodmeal increased gradually reaching a peak at 72-120 h after the bloodmeal, and then decreased...

Studies on the Midgut Lectin Gene of Glossina Austeni

Abstract: Trypanosomiasis continues to be a serious problem in sub-Saharan Africa. The inadequacies of existing control strategies necessitate the development of alternative control measures. In most cases, the causative agents of African trypanosomiasis, Trypanosoma spp., require an obligatory passage through the tsetse, Glossina spp (Diptera; Glossinidae ), vector. This developmental step of the parasite leads to vector-parasite interactions, especially within the midgut. Among the vector ...

Gene Expression Profiling of Odorant Binding Proteins in the Tsetse fly Glossina brevipalpis

Abstract: Glossina brevipalpis is a vector of trypanosomes that cause Animal African Trypanosomiasis. Chemoreception in tsetse flies is critical for identifying larvipositioning sites, hosts and mates. Odorant binding proteins (OBPs) are thought to mediate insect chemoreception by shuttling odours to receptors on olfactory sensory neurons. Little is known about the roles of OBPs in tsetse fly chemoreception. The genome of G. brevipalpis has been sequenced and 28 OBPs identified. This study a...

Genetic Variation in Two Populations of the Brown Ear Tick, Rhipicephalus Appendiculatus, in Kenya

Abstract: Several studies have shown that Rhipicephalus appendiculatus from geographically isolated areas differ in their susceptibilities to Theileria parva, the haemoprotozoan causative agent of East Coast Fever. In Kenya, R appendiculatus from Muguga have been reported to be less susceptible to T. parva infection than those from Rusinga Islands. As the same tick species from different areas shows variation in vector competence, it was considered to be of epidemiological interest to determ...

DNA Barcoding and Morphometrics of Stingless Bees (apidae: meliponinae) in three selected forests in Kenya

Abstract: Stingless honeybee keeping is an income generating enterprise with an indirect potential of achieving the goal of forest and biodiversity conservation in Kenya. However, little information is available on species diversity and spatial distribution of stingless bees in Kenya. This study describes variations of stingless bee samples collected from Arabuko Sokoke, Mwingi and Kakamega forests, ranging from low, middle to high altitude, respectively using morphometrics and DNA barcoding...

Biochemical Alterations in Maize Plants induced by Viruses causing Maize Lethal Necrosis and their Relevance for Insect Vectors

Abstract: Maize lethal necrosis (MLN) - a big threat to maize production and food security in Kenya, is caused by co-infection of maize with Maize chlorotic mottle virus (MCMV) and Sugarcane mosaic virus (SCMV). In severely affected maize fields, MLN destroys the crop completely with a devastating impact on food security of smallholder households. Since the disease was only recently documented in Kenya, there is limited information on its pathogenesis due to SCMV and MCMV interaction, the ef...


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